Anxiety-like behavior in mice in two apparatuses during withdrawal from chronic ethanol vapor inhalation

C. L. Kliethermes, K. Cronise, John Jr Crabbe

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Background: Anxiety during ethanol withdrawal may be a factor in relapse to alcohol abuse and dependence. Animal models of ethanol withdrawal have typically used forced consumption of an ethanol-containing liquid diet to induce dependence. Ethanol vapor inhalation offers an advantage over liquid diet consumption in that the onset of withdrawal can be temporally controlled more precisely, allowing studies of the development of withdrawal symptoms. Methods: The purpose of the current study was to induce ethanol dependence in mice using an inhalation procedure and to assess withdrawal anxiety symptoms behaviorally in the elevated zero maze and in the light/dark box. Male and female mice were exposed to 3 days of ethanol vapors. Anxiety-like behavior was measured on the elevated zero maze and light/dark box at multiple time points during withdrawal. Results: Mice experiencing ethanol withdrawal demonstrated increased anxiety-like behaviors relative to control animals in both apparatuses. However, this finding was specific to the procedure used with the elevated zero maze and was strongly influenced by sex in the light/dark box. Conclusions: Ethanol vapor inhalation appears to be a valid tool for the study of withdrawal-induced anxiety.

Original languageEnglish (US)
Pages (from-to)1012-1019
Number of pages8
JournalAlcoholism: Clinical and Experimental Research
Volume28
Issue number7
DOIs
StatePublished - Jul 2004

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Inhalation
Ethanol
Anxiety
Vapors
Substance Withdrawal Syndrome
Nutrition
Light
Alcoholism
Animals
Diet
Liquids
Animal Models
Alcohols
Recurrence

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Anxiety-like behavior in mice in two apparatuses during withdrawal from chronic ethanol vapor inhalation. / Kliethermes, C. L.; Cronise, K.; Crabbe, John Jr.

In: Alcoholism: Clinical and Experimental Research, Vol. 28, No. 7, 07.2004, p. 1012-1019.

Research output: Contribution to journalArticle

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