Antiviral susceptibilities and analysis of UL97 and DNA polymerase sequences of clinical cytomegalovirus isolates from immunocompromised patients

Alejo Erice, Cristina Gil-Roda, José Luis Pérez, Henry H. Balfour, Kim J. Sannerud, Michelle N. Hanson, Guy Boivin, Sunwen Chou

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Abstract

Antiviral susceptibilities to ganciclovir, foscarnet, and cidofovir and sequencing of UL97 and DNA polymerase were done on 23 cytomegalovirus (CMV) isolates from 10 immunocompromised persons with end-organ CMV disease who were treated with ganciclovir alone or ganciclovir followed by foscarnet. Screening of UL97 for ganciclovir resistance mutations was done by restriction digest analysis. Of 14 isolates resistant to ganciclovir, 11 (79%) contained one or more UL97 mutations at codons known to confer resistance to this compound, and 10 (91%) had a concordant mutant pattern by restriction digest analysis. Of 9 isolates containing mutations in conserved regions of the DNA polymerase, 8 were resistant to ganciclovir, and 4 were cross-resistant to cidofovir. All isolates were susceptible to foscarnet. It is concluded that ganciclovir-resistant clinical CMV isolates may contain UL97 mutations, DNA polymerase mutations, or mutations in both genes. Ganciclovir therapy may select for CMV isolates that are cross-resistant to cidofovir.

Original languageEnglish (US)
Pages (from-to)1087-1092
Number of pages6
JournalJournal of Infectious Diseases
Volume175
Issue number5
Publication statusPublished - 1997
Externally publishedYes

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ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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