Antiproliferative effect of retinoic acid in intravitreous silicone oil in an animal model of proliferative vitreoretinopathy

J. J. Araiz, M. F. Refojo, M. H. Arroyo, F. L. Leong, Daniel Albert, F. I. Tolentino

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Purpose. To evaluate, in vitro, the solubility and stability of all-trans RA in silicone oil (SiO) and, in vivo, the stability and the antiproliferative effect of all-trans RA in SiO on an experimental model of PVR. Methods. The solubility and stability of RA in SiO, in vitro and in vivo, were evaluated by HPLC. Rabbits underwent unilateral gas-compression vitrectomy and gas-SiO exchange. Rabbits received 10 μg (n = 17), 5 μg (n = 11), and 2 μg (n = 9) of all-trans RA in SiO, and SiO only (n = 12). All rabbits received an intravitreous injection of 150,000 fibroblasts. Results. RA is stable in SiO in vitro, but some isomerization from all-trans to 13- cis was observed under light exposure. In vivo, after 1 week, trace amounts of RA in SiO were observed in the controls and in the experimental animals, suggesting a steady state between the release of RA from the SiO and from the retina to the SiO. The rate of tractional retinal detachment was significantly lower in the animals that received 10 and 5 μg of RA than in the controls (P < 0.05). No statistical differences were found between the eyes treated with 10 and 5 μg of RA. Eyes that received 2 μg of RA showed no difference from the control group. Conclusions. The in vivo data suggest that retinoic acid might be useful as an antiproliferative agent in human eyes.

Original languageEnglish (US)
Pages (from-to)522-530
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume34
Issue number3
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Proliferative Vitreoretinopathy
Silicone Oils
Tretinoin
Animal Models
Rabbits
Solubility
Gases
Vitrectomy
Retinal Detachment
Retina
Theoretical Models
Fibroblasts
High Pressure Liquid Chromatography

Keywords

  • antiproliferative
  • proliferative
  • retinoic acid
  • silicone oil
  • vitamin A
  • vitreoretinopathy

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Antiproliferative effect of retinoic acid in intravitreous silicone oil in an animal model of proliferative vitreoretinopathy. / Araiz, J. J.; Refojo, M. F.; Arroyo, M. H.; Leong, F. L.; Albert, Daniel; Tolentino, F. I.

In: Investigative Ophthalmology and Visual Science, Vol. 34, No. 3, 01.01.1993, p. 522-530.

Research output: Contribution to journalArticle

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abstract = "Purpose. To evaluate, in vitro, the solubility and stability of all-trans RA in silicone oil (SiO) and, in vivo, the stability and the antiproliferative effect of all-trans RA in SiO on an experimental model of PVR. Methods. The solubility and stability of RA in SiO, in vitro and in vivo, were evaluated by HPLC. Rabbits underwent unilateral gas-compression vitrectomy and gas-SiO exchange. Rabbits received 10 μg (n = 17), 5 μg (n = 11), and 2 μg (n = 9) of all-trans RA in SiO, and SiO only (n = 12). All rabbits received an intravitreous injection of 150,000 fibroblasts. Results. RA is stable in SiO in vitro, but some isomerization from all-trans to 13- cis was observed under light exposure. In vivo, after 1 week, trace amounts of RA in SiO were observed in the controls and in the experimental animals, suggesting a steady state between the release of RA from the SiO and from the retina to the SiO. The rate of tractional retinal detachment was significantly lower in the animals that received 10 and 5 μg of RA than in the controls (P < 0.05). No statistical differences were found between the eyes treated with 10 and 5 μg of RA. Eyes that received 2 μg of RA showed no difference from the control group. Conclusions. The in vivo data suggest that retinoic acid might be useful as an antiproliferative agent in human eyes.",
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N2 - Purpose. To evaluate, in vitro, the solubility and stability of all-trans RA in silicone oil (SiO) and, in vivo, the stability and the antiproliferative effect of all-trans RA in SiO on an experimental model of PVR. Methods. The solubility and stability of RA in SiO, in vitro and in vivo, were evaluated by HPLC. Rabbits underwent unilateral gas-compression vitrectomy and gas-SiO exchange. Rabbits received 10 μg (n = 17), 5 μg (n = 11), and 2 μg (n = 9) of all-trans RA in SiO, and SiO only (n = 12). All rabbits received an intravitreous injection of 150,000 fibroblasts. Results. RA is stable in SiO in vitro, but some isomerization from all-trans to 13- cis was observed under light exposure. In vivo, after 1 week, trace amounts of RA in SiO were observed in the controls and in the experimental animals, suggesting a steady state between the release of RA from the SiO and from the retina to the SiO. The rate of tractional retinal detachment was significantly lower in the animals that received 10 and 5 μg of RA than in the controls (P < 0.05). No statistical differences were found between the eyes treated with 10 and 5 μg of RA. Eyes that received 2 μg of RA showed no difference from the control group. Conclusions. The in vivo data suggest that retinoic acid might be useful as an antiproliferative agent in human eyes.

AB - Purpose. To evaluate, in vitro, the solubility and stability of all-trans RA in silicone oil (SiO) and, in vivo, the stability and the antiproliferative effect of all-trans RA in SiO on an experimental model of PVR. Methods. The solubility and stability of RA in SiO, in vitro and in vivo, were evaluated by HPLC. Rabbits underwent unilateral gas-compression vitrectomy and gas-SiO exchange. Rabbits received 10 μg (n = 17), 5 μg (n = 11), and 2 μg (n = 9) of all-trans RA in SiO, and SiO only (n = 12). All rabbits received an intravitreous injection of 150,000 fibroblasts. Results. RA is stable in SiO in vitro, but some isomerization from all-trans to 13- cis was observed under light exposure. In vivo, after 1 week, trace amounts of RA in SiO were observed in the controls and in the experimental animals, suggesting a steady state between the release of RA from the SiO and from the retina to the SiO. The rate of tractional retinal detachment was significantly lower in the animals that received 10 and 5 μg of RA than in the controls (P < 0.05). No statistical differences were found between the eyes treated with 10 and 5 μg of RA. Eyes that received 2 μg of RA showed no difference from the control group. Conclusions. The in vivo data suggest that retinoic acid might be useful as an antiproliferative agent in human eyes.

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