Antigen-specific accumulation of naïve, memory and effector CD4 T cells during anterior uveitis monitored by intravital microscopy

Per A. Dullforce, Greg W. Seitz, Kiera L. Garman, Julie A. Michael, Sergio M. Crespo, Ross J. Fleischman, Stephen R. Planck, David C. Parker, James T. Rosenbaum

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Uveitis is an immune-mediated ocular disease and a leading cause of blindness. We characterized a novel model of uveitis with intravital microscopy. Transfer of ovalbumin-specific T cells from DO11.10 spleen to BALB/c recipients and subsequent challenge with ovalbumin in the anterior chamber of the eye resulted in anterior uveitis. Antigen-specificity was verified by injection of irrelevant antigen and transfer of T cells with a different specificity. Subsets of CD4 T cells, including naive (DO11.10 RAG-/-) and in vitro-activated Th2 effector CD4 T cells, infiltrated anterior segment tissues early in the inflammation. Memory-like CD44high CD4 T cells from unprimed transgenic mice and in vitro-activated Th1 effector CD4 T cells accumulated to larger numbers than naive or Th2 effector cells at 48 and 72 h. Of these, the α2-integrin + CD4 unprimed T cells entered the eye more efficiently, and antibody to α2-integrin markedly inhibited the inflammatory response. Intravital microscopy revealed the early arrival and antigen-specific accumulation of CD4 T cells in inflamed tissue and should be helpful in understanding T cell migration to other organs.

Original languageEnglish (US)
Pages (from-to)49-60
Number of pages12
JournalCellular Immunology
Volume239
Issue number1
DOIs
StatePublished - Jan 2006

Keywords

  • Cell trafficking
  • Inflammation
  • Intravital microscopy
  • T cells

ASJC Scopus subject areas

  • Immunology

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