Abstract
Uveitis is an immune-mediated ocular disease and a leading cause of blindness. We characterized a novel model of uveitis with intravital microscopy. Transfer of ovalbumin-specific T cells from DO11.10 spleen to BALB/c recipients and subsequent challenge with ovalbumin in the anterior chamber of the eye resulted in anterior uveitis. Antigen-specificity was verified by injection of irrelevant antigen and transfer of T cells with a different specificity. Subsets of CD4 T cells, including naive (DO11.10 RAG-/-) and in vitro-activated Th2 effector CD4 T cells, infiltrated anterior segment tissues early in the inflammation. Memory-like CD44high CD4 T cells from unprimed transgenic mice and in vitro-activated Th1 effector CD4 T cells accumulated to larger numbers than naive or Th2 effector cells at 48 and 72 h. Of these, the α2-integrin + CD4 unprimed T cells entered the eye more efficiently, and antibody to α2-integrin markedly inhibited the inflammatory response. Intravital microscopy revealed the early arrival and antigen-specific accumulation of CD4 T cells in inflamed tissue and should be helpful in understanding T cell migration to other organs.
Original language | English (US) |
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Pages (from-to) | 49-60 |
Number of pages | 12 |
Journal | Cellular Immunology |
Volume | 239 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2006 |
Keywords
- Cell trafficking
- Inflammation
- Intravital microscopy
- T cells
ASJC Scopus subject areas
- Immunology