Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis

P. J. Horner, M. Ali, David Parker, J. N. Weber, D. Taylor-Robinson, M. O. McClure

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims - To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. Methods - The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a β-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against β-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development on addition of peroxidase substrate, antibody recognition of antigen was quantified by optical density at 492 nm. Results - A sensitive and relatively specific ELISA to detect hsp60 has been produced, which can be exploited to determine the antibody response to C trachomatis hsp60. Conclusions - This assay will permit the future investigation of the immunopathogenesis of persistent inflammation following C trachomatis infection.

Original languageEnglish (US)
Pages (from-to)642-647
Number of pages6
JournalJournal of Clinical Pathology
Volume49
Issue number8
StatePublished - 1996
Externally publishedYes

Fingerprint

Galactosidases
Chlamydia trachomatis
Heat-Shock Proteins
Peroxidase
Enzyme-Linked Immunosorbent Assay
Antigens
Recombinant Proteins
Goats
Antibody Formation
Anti-Idiotypic Antibodies
Color
Monoclonal Antibodies
Escherichia coli
Inflammation
Polymerase Chain Reaction
Antibodies
Infection
Genes

Keywords

  • Antibody
  • Chlamydia trachomatis
  • ELISA
  • Heat shock protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Horner, P. J., Ali, M., Parker, D., Weber, J. N., Taylor-Robinson, D., & McClure, M. O. (1996). Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis. Journal of Clinical Pathology, 49(8), 642-647.

Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis. / Horner, P. J.; Ali, M.; Parker, David; Weber, J. N.; Taylor-Robinson, D.; McClure, M. O.

In: Journal of Clinical Pathology, Vol. 49, No. 8, 1996, p. 642-647.

Research output: Contribution to journalArticle

Horner, PJ, Ali, M, Parker, D, Weber, JN, Taylor-Robinson, D & McClure, MO 1996, 'Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis', Journal of Clinical Pathology, vol. 49, no. 8, pp. 642-647.
Horner PJ, Ali M, Parker D, Weber JN, Taylor-Robinson D, McClure MO. Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis. Journal of Clinical Pathology. 1996;49(8):642-647.
Horner, P. J. ; Ali, M. ; Parker, David ; Weber, J. N. ; Taylor-Robinson, D. ; McClure, M. O. / Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis. In: Journal of Clinical Pathology. 1996 ; Vol. 49, No. 8. pp. 642-647.
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