Abstract
Aims - To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. Methods - The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a β-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against β-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development on addition of peroxidase substrate, antibody recognition of antigen was quantified by optical density at 492 nm. Results - A sensitive and relatively specific ELISA to detect hsp60 has been produced, which can be exploited to determine the antibody response to C trachomatis hsp60. Conclusions - This assay will permit the future investigation of the immunopathogenesis of persistent inflammation following C trachomatis infection.
Original language | English (US) |
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Pages (from-to) | 642-647 |
Number of pages | 6 |
Journal | Journal of Clinical Pathology |
Volume | 49 |
Issue number | 8 |
DOIs | |
State | Published - 1996 |
Externally published | Yes |
Keywords
- Antibody
- Chlamydia trachomatis
- ELISA
- Heat shock protein
ASJC Scopus subject areas
- Pathology and Forensic Medicine