Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis

P. J. Horner, M. Ali, D. Parker, J. N. Weber, D. Taylor-Robinson, M. O. McClure

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Aims - To develop an indirect ELISA using the heat shock protein (hsp60) of Chlamydia trachomatis as antigen. Methods - The hsp60 gene was amplified by PCR, expressed in the vector pDEV-107 and transformed into Escherichia coli. The recombinant protein, expressed as a β-galactosidase fusion product, was captured onto a solid phase using a monoclonal antibody directed against β-galactosidase. Following incubation with goat anti-human antibody conjugated to peroxidase and colour development on addition of peroxidase substrate, antibody recognition of antigen was quantified by optical density at 492 nm. Results - A sensitive and relatively specific ELISA to detect hsp60 has been produced, which can be exploited to determine the antibody response to C trachomatis hsp60. Conclusions - This assay will permit the future investigation of the immunopathogenesis of persistent inflammation following C trachomatis infection.

Original languageEnglish (US)
Pages (from-to)642-647
Number of pages6
JournalJournal of Clinical Pathology
Volume49
Issue number8
DOIs
StatePublished - Jan 1 1996

Keywords

  • Antibody
  • Chlamydia trachomatis
  • ELISA
  • Heat shock protein

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Horner, P. J., Ali, M., Parker, D., Weber, J. N., Taylor-Robinson, D., & McClure, M. O. (1996). Antigen capture ELISA for the heat shock protein (hsp60) of Chlamydia trachomatis. Journal of Clinical Pathology, 49(8), 642-647. https://doi.org/10.1136/jcp.49.8.642