Abstract
In human immunodeficiency virus type 1 (HIV-I) infected individuals, the antibody response to the external envelope (gpl20) is associated with in vitro neutralization. To further characterize the anti-gpl20 response, we examined the IgG reactivity of 75 HIV-1-seroconverted and 200 HIV-1-seropositive individuals to deletion mutants of gpl20 in an enzyme immunoassay. We used yeast-derived, non-glycosylated recombinant HIV-1 SF2 gpl20 equivalent and-variants deleted in variable regions. We observed two distinctive response patterns: IgG non-responders (SF2-V3-restricted responders) and IgG responders to conserved regions of gp!20. This divergence in response pattern occurred soon after gag/env HIV-1 antibody seroconversion and persisted in time within an individual. In addition, the SF2-V3-restricted responders had a higher frequency of HIV-1 core antigen positivity and HIV-1 core antibody negativity than the non-restricted responders. These results suggest that specific and persistent host antibody response patterns to gpl20 develop early in HIV-1 infection and that these patterns are associated with differences in HIV-1 expression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 160-172 |
| Number of pages | 13 |
| Journal | Intervirology |
| Volume | 32 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1991 |
| Externally published | Yes |
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Keywords
- GPL20
- HIV-1 expression
- HIV-1 SF2
- IgG
- V3-neutralizing epitope
- Variable region
ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope. / Back, Nicole K T; Haigwood, Nancy; De Wolf, Frank; De Jongh, Barlelt M.; Goudsmit, Jaap.
In: Intervirology, Vol. 32, No. 3, 1991, p. 160-172.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope
AU - Back, Nicole K T
AU - Haigwood, Nancy
AU - De Wolf, Frank
AU - De Jongh, Barlelt M.
AU - Goudsmit, Jaap
PY - 1991
Y1 - 1991
N2 - In human immunodeficiency virus type 1 (HIV-I) infected individuals, the antibody response to the external envelope (gpl20) is associated with in vitro neutralization. To further characterize the anti-gpl20 response, we examined the IgG reactivity of 75 HIV-1-seroconverted and 200 HIV-1-seropositive individuals to deletion mutants of gpl20 in an enzyme immunoassay. We used yeast-derived, non-glycosylated recombinant HIV-1 SF2 gpl20 equivalent and-variants deleted in variable regions. We observed two distinctive response patterns: IgG non-responders (SF2-V3-restricted responders) and IgG responders to conserved regions of gp!20. This divergence in response pattern occurred soon after gag/env HIV-1 antibody seroconversion and persisted in time within an individual. In addition, the SF2-V3-restricted responders had a higher frequency of HIV-1 core antigen positivity and HIV-1 core antibody negativity than the non-restricted responders. These results suggest that specific and persistent host antibody response patterns to gpl20 develop early in HIV-1 infection and that these patterns are associated with differences in HIV-1 expression.
AB - In human immunodeficiency virus type 1 (HIV-I) infected individuals, the antibody response to the external envelope (gpl20) is associated with in vitro neutralization. To further characterize the anti-gpl20 response, we examined the IgG reactivity of 75 HIV-1-seroconverted and 200 HIV-1-seropositive individuals to deletion mutants of gpl20 in an enzyme immunoassay. We used yeast-derived, non-glycosylated recombinant HIV-1 SF2 gpl20 equivalent and-variants deleted in variable regions. We observed two distinctive response patterns: IgG non-responders (SF2-V3-restricted responders) and IgG responders to conserved regions of gp!20. This divergence in response pattern occurred soon after gag/env HIV-1 antibody seroconversion and persisted in time within an individual. In addition, the SF2-V3-restricted responders had a higher frequency of HIV-1 core antigen positivity and HIV-1 core antibody negativity than the non-restricted responders. These results suggest that specific and persistent host antibody response patterns to gpl20 develop early in HIV-1 infection and that these patterns are associated with differences in HIV-1 expression.
KW - GPL20
KW - HIV-1 expression
KW - HIV-1 SF2
KW - IgG
KW - V3-neutralizing epitope
KW - Variable region
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U2 - 10.1159/000150196
DO - 10.1159/000150196
M3 - Article
VL - 32
SP - 160
EP - 172
JO - Intervirology
JF - Intervirology
SN - 0300-5526
IS - 3
ER -