Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope

Nicole K T Back, Nancy Haigwood, Frank De Wolf, Barlelt M. De Jongh, Jaap Goudsmit

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In human immunodeficiency virus type 1 (HIV-I) infected individuals, the antibody response to the external envelope (gpl20) is associated with in vitro neutralization. To further characterize the anti-gpl20 response, we examined the IgG reactivity of 75 HIV-1-seroconverted and 200 HIV-1-seropositive individuals to deletion mutants of gpl20 in an enzyme immunoassay. We used yeast-derived, non-glycosylated recombinant HIV-1 SF2 gpl20 equivalent and-variants deleted in variable regions. We observed two distinctive response patterns: IgG non-responders (SF2-V3-restricted responders) and IgG responders to conserved regions of gp!20. This divergence in response pattern occurred soon after gag/env HIV-1 antibody seroconversion and persisted in time within an individual. In addition, the SF2-V3-restricted responders had a higher frequency of HIV-1 core antigen positivity and HIV-1 core antibody negativity than the non-restricted responders. These results suggest that specific and persistent host antibody response patterns to gpl20 develop early in HIV-1 infection and that these patterns are associated with differences in HIV-1 expression.

Original languageEnglish (US)
Pages (from-to)160-172
Number of pages13
JournalIntervirology
Volume32
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

HIV-1
Antibodies
Immunoglobulin G
Antibody Formation
HIV Antibodies
Immunoenzyme Techniques
HIV Infections
Yeasts
Antigens

Keywords

  • GPL20
  • HIV-1 expression
  • HIV-1 SF2
  • IgG
  • V3-neutralizing epitope
  • Variable region

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Back, N. K. T., Haigwood, N., De Wolf, F., De Jongh, B. M., & Goudsmit, J. (1991). Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope. Intervirology, 32(3), 160-172. https://doi.org/10.1159/000150196

Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope. / Back, Nicole K T; Haigwood, Nancy; De Wolf, Frank; De Jongh, Barlelt M.; Goudsmit, Jaap.

In: Intervirology, Vol. 32, No. 3, 1991, p. 160-172.

Research output: Contribution to journalArticle

Back, NKT, Haigwood, N, De Wolf, F, De Jongh, BM & Goudsmit, J 1991, 'Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope', Intervirology, vol. 32, no. 3, pp. 160-172. https://doi.org/10.1159/000150196
Back, Nicole K T ; Haigwood, Nancy ; De Wolf, Frank ; De Jongh, Barlelt M. ; Goudsmit, Jaap. / Antibody reactivity to deletion mutants of the HIV-1 SF2 envelope. In: Intervirology. 1991 ; Vol. 32, No. 3. pp. 160-172.
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