Antibody reactive with Actinobacillus actinomycetemcomitans leukotoxin in early-onset periodontitis patients

Joseph Califano, B. E. Pace, J. C. Gunsolley, H. A. Schenkein, E. T. Lally, J. G. Tew

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The objective of this study was to determine whether a relationship exists between antibody reactive with the Actinobacillus actinomycetemcomitans leukotoxin and the severity of periodontal disease. Serum concentrations of antibody reactive with the leukotoxin were determined for 119 early-onset periodontitis patients and 59 non-periodontitis subjects using limiting dilution analysis on Western blots. Immunoglobulin G (IgG) antibody reactive with the A. actinomycetemcomitans leukotoxin ranged from undetectable to 29 μg/ml (mean = 3.13±0.97 μg/ml for the generalized early-onset periodontitis and 2.17±0.86 μg/ml for the localized juvenile periodontitis patients vs 0.32±0.24 ng/ml for 59 non-periodontitis controls), and the dominant subclass was IgGl. Analysis of the relationship between antibody reactive with A. actinomycetemcomitans sonicate, A. actinomycetemcomitans leukotoxin and attachment loss patterns indicates that seropositive generalized early-onset periodontitis patients had decreased attachment loss compared with patients lacking this antibody. The statistical relationship appeared to be stronger for the sonicate than the purified leukotoxin. These data suggest that antibody reactive with A. actinomycetemcomitans leukotoxin may be protective in early-onset periodontitis, but given that the sonicate appeared better than the leukotoxin alone, it is not likely that leukotoxin is the only antigen of importance to host defense.

Original languageEnglish (US)
Pages (from-to)20-26
Number of pages7
JournalOral Microbiology and Immunology
Volume12
Issue number1
StatePublished - Feb 1997
Externally publishedYes

Fingerprint

Aggressive Periodontitis
Aggregatibacter actinomycetemcomitans
Antibodies
leukotoxin
Periodontal Diseases
Immunoglobulin G
Western Blotting
Antigens

Keywords

  • Actinobacillus actinomycetemcomitans
  • Antibody
  • Attachment loss
  • Early-onset periodontitis
  • Leukotoxin

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Califano, J., Pace, B. E., Gunsolley, J. C., Schenkein, H. A., Lally, E. T., & Tew, J. G. (1997). Antibody reactive with Actinobacillus actinomycetemcomitans leukotoxin in early-onset periodontitis patients. Oral Microbiology and Immunology, 12(1), 20-26.

Antibody reactive with Actinobacillus actinomycetemcomitans leukotoxin in early-onset periodontitis patients. / Califano, Joseph; Pace, B. E.; Gunsolley, J. C.; Schenkein, H. A.; Lally, E. T.; Tew, J. G.

In: Oral Microbiology and Immunology, Vol. 12, No. 1, 02.1997, p. 20-26.

Research output: Contribution to journalArticle

Califano, Joseph ; Pace, B. E. ; Gunsolley, J. C. ; Schenkein, H. A. ; Lally, E. T. ; Tew, J. G. / Antibody reactive with Actinobacillus actinomycetemcomitans leukotoxin in early-onset periodontitis patients. In: Oral Microbiology and Immunology. 1997 ; Vol. 12, No. 1. pp. 20-26.
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