TY - JOUR
T1 - Antibody combining site heterogeneity within the response to phosphocholine-keyhole limpet hemocyanin
AU - Bruderer, Urs
AU - Stenzel-Poore, Mary P.
AU - Bächinger, Hans Peter
AU - Fellman, Jack H.
AU - Rittenberg, Marvin B.
N1 - Funding Information:
*Supported in part by Grant AI 14985 from the National Institutes of Health. IlCorresoondence should be sent to: U. Bruderer, Depart-ment of Microbiology and Immunology, L220, Oregon Health Sciences University, 3181 S.W. Sam Jackson Park Road, Portland, OR 97201, U.S.A. U.B. was supported by a Swiss National Sciences Foundation Fellowship and a Tartar Foundation Research award. Abbreviations: APPC, p-aminophenyl phosphocholine; DPPC, N-(2,4-dinitrophenyl)-p-aminophenyl phospho-choline; ELISA, enzyme-linked immunosorbent assay; NPDBP, p-nitrophenyl-3,3-dimethyl butyl phosphate; NPMPC. p-nitrophenyl methyl phosphonate choline; NPEP. p-nitrophenyl ethyl phosphate; NPP, p-nitro-phenyl phosphate; NPPC, p-nitrophenyl phospho-choline; PC, phosphocholine; PC-KLH, phospho-choline-keyhole limpet hemocyanin.
PY - 1989/1
Y1 - 1989/1
N2 - The memory response to PC-KLH is dominated by two antibody populations differing in fine specificity. Group I antibodies show affinity for both phosphocholine (PC) and p-nitrophenyl phosphocholine (NPPC). Group II antibodies exhibit significant affinity only for NPPC. Here, we describe the binding site charactersitics of Group II antibodies and show that in recognizing NPPC these antibodies have a common requirement for the phenyl moiety, a negatively charged phosphate, and the trimethyl structure of the choline. However, Group II antibodies were found to differ in their requirement for the positively charged nitrogen of choline and thus could be divided into two subgroups. In contrast to Group II-A, Group II-B antibodies recognize not only NPPC but also its analog p-nitrophenyl-3,3-dimethyl butyl phosphate (NPDBP), which differs from NPPC by substituting a carbon for the positively charged nitrogen of the choline moiety. These results suggest that Group II-B antibodies do not require the positive charge in order to bind, although the binding constant, Ka, was increased when the nitrogen was present. Furthermore, heterogeneity within Group II antibodies was characterized by differences in binding to clinitrophenyl phosphocholine which has an additional phenyl ring and aminophenyl phosphocholine which has an amino group in place of the nitro group of NPPC. The results indicate that diversity in the memory response to PC-KLH is reflected in the Group II antigen-binding phenotype by antibodies which differ appreciably in their recognition of various structural aspects of the hapten.
AB - The memory response to PC-KLH is dominated by two antibody populations differing in fine specificity. Group I antibodies show affinity for both phosphocholine (PC) and p-nitrophenyl phosphocholine (NPPC). Group II antibodies exhibit significant affinity only for NPPC. Here, we describe the binding site charactersitics of Group II antibodies and show that in recognizing NPPC these antibodies have a common requirement for the phenyl moiety, a negatively charged phosphate, and the trimethyl structure of the choline. However, Group II antibodies were found to differ in their requirement for the positively charged nitrogen of choline and thus could be divided into two subgroups. In contrast to Group II-A, Group II-B antibodies recognize not only NPPC but also its analog p-nitrophenyl-3,3-dimethyl butyl phosphate (NPDBP), which differs from NPPC by substituting a carbon for the positively charged nitrogen of the choline moiety. These results suggest that Group II-B antibodies do not require the positive charge in order to bind, although the binding constant, Ka, was increased when the nitrogen was present. Furthermore, heterogeneity within Group II antibodies was characterized by differences in binding to clinitrophenyl phosphocholine which has an additional phenyl ring and aminophenyl phosphocholine which has an amino group in place of the nitro group of NPPC. The results indicate that diversity in the memory response to PC-KLH is reflected in the Group II antigen-binding phenotype by antibodies which differ appreciably in their recognition of various structural aspects of the hapten.
UR - http://www.scopus.com/inward/record.url?scp=0024553561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024553561&partnerID=8YFLogxK
U2 - 10.1016/0161-5890(89)90021-7
DO - 10.1016/0161-5890(89)90021-7
M3 - Article
C2 - 2467198
AN - SCOPUS:0024553561
SN - 0161-5890
VL - 26
SP - 63
EP - 71
JO - Molecular Immunology
JF - Molecular Immunology
IS - 1
ER -