Antibodies Induced by a Primary Cytomegalovirus Infection React with Human Herpesvirus 6 Proteins

Stuart P. Adler; Michael McVoy; Sunwen Chou; Sue Hempfling; Koichi Yamanishi; William Britt

doi:10.1093/infdis/168.5.1119

Antibodies Induced by a Primary Cytomegalovirus Infection React with Human Herpesvirus 6 Proteins

Stuart P. Adler, Michael McVoy, Sunwen Chou, Sue Hempfling, Koichi Yamanishi, William Britt

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

After a primary human cytomegalovirus (HCMV) infection, antibody titer to human herpesvirus 6 (HHV-6) rises. To determine if this occurs because of simultaneous infection with both viruses, serologic responses to these viruses were investigated among healthy women who received a live HCMV vaccine or acquired HCMV from an infected child. Both vaccination and natural infection caused four-fold or greater titer rises to HHV-6. Analysis of sera from 5 children with primary HHV-6 showed no serologic response to HCMV. Absorption of 7 sera with HCMV antigens reduced titers to HHV-6 by two- to fourfold. Immunoblot analysis of sera obtained from 10 persons before and after a primary HCMV infection revealed that after HCMV infection, 2 persons developed IgG reactivity to the 116-kDa protein (gp116) of HHV-6 and to two cleavage products of this protein. Reactivity to HHV-6 gp116 appeared after HCMV seroconversion and was removed by absorption of sera with HCMV glycoprotein gB (gB), indicating that antibodies to HCMV gB cross-react with HHV-6 gp116, the likely gB homologue of HHV6. The serologic diagnosis of HHV-6 infection requires excluding a primary HCMV infection.

Original language	English (US)
Pages (from-to)	1119-1126
Number of pages	8
Journal	Journal of Infectious Diseases
Volume	168
Issue number	5
DOIs	https://doi.org/10.1093/infdis/168.5.1119
State	Published - Dec 1993
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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@article{db6962f83e4a4476bdd900284358790a,

title = "Antibodies Induced by a Primary Cytomegalovirus Infection React with Human Herpesvirus 6 Proteins",

abstract = "After a primary human cytomegalovirus (HCMV) infection, antibody titer to human herpesvirus 6 (HHV-6) rises. To determine if this occurs because of simultaneous infection with both viruses, serologic responses to these viruses were investigated among healthy women who received a live HCMV vaccine or acquired HCMV from an infected child. Both vaccination and natural infection caused four-fold or greater titer rises to HHV-6. Analysis of sera from 5 children with primary HHV-6 showed no serologic response to HCMV. Absorption of 7 sera with HCMV antigens reduced titers to HHV-6 by two- to fourfold. Immunoblot analysis of sera obtained from 10 persons before and after a primary HCMV infection revealed that after HCMV infection, 2 persons developed IgG reactivity to the 116-kDa protein (gp116) of HHV-6 and to two cleavage products of this protein. Reactivity to HHV-6 gp116 appeared after HCMV seroconversion and was removed by absorption of sera with HCMV glycoprotein gB (gB), indicating that antibodies to HCMV gB cross-react with HHV-6 gp116, the likely gB homologue of HHV6. The serologic diagnosis of HHV-6 infection requires excluding a primary HCMV infection.",

author = "Adler, {Stuart P.} and Michael McVoy and Sunwen Chou and Sue Hempfling and Koichi Yamanishi and William Britt",

note = "Funding Information: Received 30 March 1993; revised 28 June 1993. Presented in part: Society for Pediatric Research. Baltimore. 1992. Informed consent was obtained from all subjects. The human experimentation guidelines of the US Department of Health and Human Services and those of Virginia Commonwealth University and Medical College of Virginia were followed. Grant support: National Institutes ofHealth. Reprints or correspondence: Dr. Stuart P. Adler. Medical College of Virginia. Box 163. Richmond. VA 23298-0163.",

year = "1993",

month = dec,

doi = "10.1093/infdis/168.5.1119",

language = "English (US)",

volume = "168",

pages = "1119--1126",

journal = "Journal of Infectious Diseases",

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T1 - Antibodies Induced by a Primary Cytomegalovirus Infection React with Human Herpesvirus 6 Proteins

AU - Adler, Stuart P.

AU - McVoy, Michael

AU - Chou, Sunwen

AU - Hempfling, Sue

AU - Yamanishi, Koichi

AU - Britt, William

N1 - Funding Information: Received 30 March 1993; revised 28 June 1993. Presented in part: Society for Pediatric Research. Baltimore. 1992. Informed consent was obtained from all subjects. The human experimentation guidelines of the US Department of Health and Human Services and those of Virginia Commonwealth University and Medical College of Virginia were followed. Grant support: National Institutes ofHealth. Reprints or correspondence: Dr. Stuart P. Adler. Medical College of Virginia. Box 163. Richmond. VA 23298-0163.

PY - 1993/12

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N2 - After a primary human cytomegalovirus (HCMV) infection, antibody titer to human herpesvirus 6 (HHV-6) rises. To determine if this occurs because of simultaneous infection with both viruses, serologic responses to these viruses were investigated among healthy women who received a live HCMV vaccine or acquired HCMV from an infected child. Both vaccination and natural infection caused four-fold or greater titer rises to HHV-6. Analysis of sera from 5 children with primary HHV-6 showed no serologic response to HCMV. Absorption of 7 sera with HCMV antigens reduced titers to HHV-6 by two- to fourfold. Immunoblot analysis of sera obtained from 10 persons before and after a primary HCMV infection revealed that after HCMV infection, 2 persons developed IgG reactivity to the 116-kDa protein (gp116) of HHV-6 and to two cleavage products of this protein. Reactivity to HHV-6 gp116 appeared after HCMV seroconversion and was removed by absorption of sera with HCMV glycoprotein gB (gB), indicating that antibodies to HCMV gB cross-react with HHV-6 gp116, the likely gB homologue of HHV6. The serologic diagnosis of HHV-6 infection requires excluding a primary HCMV infection.

AB - After a primary human cytomegalovirus (HCMV) infection, antibody titer to human herpesvirus 6 (HHV-6) rises. To determine if this occurs because of simultaneous infection with both viruses, serologic responses to these viruses were investigated among healthy women who received a live HCMV vaccine or acquired HCMV from an infected child. Both vaccination and natural infection caused four-fold or greater titer rises to HHV-6. Analysis of sera from 5 children with primary HHV-6 showed no serologic response to HCMV. Absorption of 7 sera with HCMV antigens reduced titers to HHV-6 by two- to fourfold. Immunoblot analysis of sera obtained from 10 persons before and after a primary HCMV infection revealed that after HCMV infection, 2 persons developed IgG reactivity to the 116-kDa protein (gp116) of HHV-6 and to two cleavage products of this protein. Reactivity to HHV-6 gp116 appeared after HCMV seroconversion and was removed by absorption of sera with HCMV glycoprotein gB (gB), indicating that antibodies to HCMV gB cross-react with HHV-6 gp116, the likely gB homologue of HHV6. The serologic diagnosis of HHV-6 infection requires excluding a primary HCMV infection.

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Antibodies Induced by a Primary Cytomegalovirus Infection React with Human Herpesvirus 6 Proteins

Abstract

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