Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4

P. Barber, Marvin Rittenberg

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Anti-trinitrophenyl antibodies are detected by the specific neutralization of bacteriophage T4 haptenated with the picryl radical (TNP). TNP-phage neutralization by anti-TNP is compared to neutralization by anti-phage. Anti-TNP neutralized haptenated phage by two separable first order processes. The initial reaction was operative until the surviving fraction approached 0·1 at which point a second, slower, apparent first order process was detectable. Neutralization of haptenated phage by anti-phage antibody appeared to proceed by a single first order reaction and thus differed from neutralization by anti-TNP antibody. Neutralization by anti-TNP is hapten specific since anti-hapten antibody was unable to neutralize native phage and since neutralization of TNP-phage by anti-TNP was inhibited by TNP-ε{lunate}-aminocaproic acid which does not affect anti-phage antibodies. Neutralization of TNP-phage by anti-hapten also differs from neutralization of TNP-phage in the rat the former is more dependent on antigen concentration. This assay was used to detect primary 19S and 7S anti-hapten antibodies separated by sucrose gradient centrifugation and provides a means of detecting the two classes of anti-TNP antibodies at very low concentration.

Original languageEnglish (US)
Pages (from-to)163-174
Number of pages12
JournalImmunochemistry
Volume6
Issue number2
StatePublished - Mar 1969

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Bacteriophage T4
Bacteriophages
Anti-Idiotypic Antibodies
Haptens
Aminocaproic Acid
Centrifugation
Sucrose
Antigens

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4. / Barber, P.; Rittenberg, Marvin.

In: Immunochemistry, Vol. 6, No. 2, 03.1969, p. 163-174.

Research output: Contribution to journalArticle

Barber, P & Rittenberg, M 1969, 'Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4', Immunochemistry, vol. 6, no. 2, pp. 163-174.
Barber, P. ; Rittenberg, Marvin. / Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4. In: Immunochemistry. 1969 ; Vol. 6, No. 2. pp. 163-174.
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