Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4

P. Barber; M. B. Rittenberg

doi:10.1016/0019-2791(69)90153-0

Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4

P. Barber, M. B. Rittenberg

Molecular Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Anti-trinitrophenyl antibodies are detected by the specific neutralization of bacteriophage T4 haptenated with the picryl radical (TNP). TNP-phage neutralization by anti-TNP is compared to neutralization by anti-phage. Anti-TNP neutralized haptenated phage by two separable first order processes. The initial reaction was operative until the surviving fraction approached 0·1 at which point a second, slower, apparent first order process was detectable. Neutralization of haptenated phage by anti-phage antibody appeared to proceed by a single first order reaction and thus differed from neutralization by anti-TNP antibody. Neutralization by anti-TNP is hapten specific since anti-hapten antibody was unable to neutralize native phage and since neutralization of TNP-phage by anti-TNP was inhibited by TNP-ε{lunate}-aminocaproic acid which does not affect anti-phage antibodies. Neutralization of TNP-phage by anti-hapten also differs from neutralization of TNP-phage in the rat the former is more dependent on antigen concentration. This assay was used to detect primary 19S and 7S anti-hapten antibodies separated by sucrose gradient centrifugation and provides a means of detecting the two classes of anti-TNP antibodies at very low concentration.

Original language	English (US)
Pages (from-to)	163-174
Number of pages	12
Journal	Immunochemistry
Volume	6
Issue number	2
DOIs	https://doi.org/10.1016/0019-2791(69)90153-0
State	Published - Mar 1969

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General Medicine

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10.1016/0019-2791(69)90153-0

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title = "Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4",

abstract = "Anti-trinitrophenyl antibodies are detected by the specific neutralization of bacteriophage T4 haptenated with the picryl radical (TNP). TNP-phage neutralization by anti-TNP is compared to neutralization by anti-phage. Anti-TNP neutralized haptenated phage by two separable first order processes. The initial reaction was operative until the surviving fraction approached 0·1 at which point a second, slower, apparent first order process was detectable. Neutralization of haptenated phage by anti-phage antibody appeared to proceed by a single first order reaction and thus differed from neutralization by anti-TNP antibody. Neutralization by anti-TNP is hapten specific since anti-hapten antibody was unable to neutralize native phage and since neutralization of TNP-phage by anti-TNP was inhibited by TNP-ε{lunate}-aminocaproic acid which does not affect anti-phage antibodies. Neutralization of TNP-phage by anti-hapten also differs from neutralization of TNP-phage in the rat the former is more dependent on antigen concentration. This assay was used to detect primary 19S and 7S anti-hapten antibodies separated by sucrose gradient centrifugation and provides a means of detecting the two classes of anti-TNP antibodies at very low concentration.",

author = "P. Barber and Rittenberg, {M. B.}",

note = "Funding Information: Coliphage T4 d+ were prepared from lysates ofE. coli B by the soft agar layer method \[5\]a, nd partially purified by differential centrifugation \[6\]A. fter dialysis *Supported by grants from the United States Public Health Service (CA-07941), The John A. Hartford Foundation, Inc. and the Medical Research Foundation of Oregon. tSummer Fellow, USPHS Training Grant HD 00165. ~tScholar of the Leukemia Society.",

year = "1969",

month = mar,

doi = "10.1016/0019-2791(69)90153-0",

language = "English (US)",

volume = "6",

pages = "163--174",

journal = "Immunochemistry",

issn = "0019-2791",

publisher = "Elsevier Limited",

number = "2",

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T1 - Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4

AU - Barber, P.

AU - Rittenberg, M. B.

N1 - Funding Information: Coliphage T4 d+ were prepared from lysates ofE. coli B by the soft agar layer method \[5\]a, nd partially purified by differential centrifugation \[6\]A. fter dialysis *Supported by grants from the United States Public Health Service (CA-07941), The John A. Hartford Foundation, Inc. and the Medical Research Foundation of Oregon. tSummer Fellow, USPHS Training Grant HD 00165. ~tScholar of the Leukemia Society.

PY - 1969/3

Y1 - 1969/3

N2 - Anti-trinitrophenyl antibodies are detected by the specific neutralization of bacteriophage T4 haptenated with the picryl radical (TNP). TNP-phage neutralization by anti-TNP is compared to neutralization by anti-phage. Anti-TNP neutralized haptenated phage by two separable first order processes. The initial reaction was operative until the surviving fraction approached 0·1 at which point a second, slower, apparent first order process was detectable. Neutralization of haptenated phage by anti-phage antibody appeared to proceed by a single first order reaction and thus differed from neutralization by anti-TNP antibody. Neutralization by anti-TNP is hapten specific since anti-hapten antibody was unable to neutralize native phage and since neutralization of TNP-phage by anti-TNP was inhibited by TNP-ε{lunate}-aminocaproic acid which does not affect anti-phage antibodies. Neutralization of TNP-phage by anti-hapten also differs from neutralization of TNP-phage in the rat the former is more dependent on antigen concentration. This assay was used to detect primary 19S and 7S anti-hapten antibodies separated by sucrose gradient centrifugation and provides a means of detecting the two classes of anti-TNP antibodies at very low concentration.

AB - Anti-trinitrophenyl antibodies are detected by the specific neutralization of bacteriophage T4 haptenated with the picryl radical (TNP). TNP-phage neutralization by anti-TNP is compared to neutralization by anti-phage. Anti-TNP neutralized haptenated phage by two separable first order processes. The initial reaction was operative until the surviving fraction approached 0·1 at which point a second, slower, apparent first order process was detectable. Neutralization of haptenated phage by anti-phage antibody appeared to proceed by a single first order reaction and thus differed from neutralization by anti-TNP antibody. Neutralization by anti-TNP is hapten specific since anti-hapten antibody was unable to neutralize native phage and since neutralization of TNP-phage by anti-TNP was inhibited by TNP-ε{lunate}-aminocaproic acid which does not affect anti-phage antibodies. Neutralization of TNP-phage by anti-hapten also differs from neutralization of TNP-phage in the rat the former is more dependent on antigen concentration. This assay was used to detect primary 19S and 7S anti-hapten antibodies separated by sucrose gradient centrifugation and provides a means of detecting the two classes of anti-TNP antibodies at very low concentration.

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JO - Immunochemistry

JF - Immunochemistry

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ER -

Anti-trinitrophenyl (TNP) antibody detection by neutralization of TNP-coliphage T4

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