TY - JOUR
T1 - Anti-rat ICAM-1 antibody does not influence the course of experimental melanin-induced uveitis
AU - Smith, J. R.
AU - O'Rourke, L. M.
AU - Becker, M. D.
AU - Cao, M.
AU - Williams, K. A.
AU - Planck, S. R.
AU - Rosenbaum, J. T.
N1 - Funding Information:
This study was supported by the National Health and Medical Research Council of Australia (JRS: 997099, KAW: 970035), National Institute of Health (JTR, SRP: EYO6484) and Research to Prevent Blindness (JTR, SRP).
PY - 2000
Y1 - 2000
N2 - Purpose. Experimental melanin-induced uveitis (EMIU) is a T cell-mediated rat model of acute anterior uveitis. We investigated the possibility of preventing the inflammation with monoclonal antibody directed against rat intercellular adhesion molecule 1 (ICAM-1). Methods. To induce EMIU, Lewis rats were immunized with bovine ocular melanin extract (250-500 μg). Each day from day 6 post-immunization, rats were injected intraperitoneally with anti-ICAM-1 monoclonal antibody (IA29) or normal mouse serum, and examined with a slit-lamp biomicroscope. On the first day of clinical inflammation, intravital microscopy of iris vasculature was performed on each animal following intraperitoneal injection of rhodamine 6G. At the peak of clinical inflammation, rats were killed, and eyes were examined histologically. Binding potency of IA29 was tested by flow cytometry using concanavalin A-stimulated rat T cells. Immunohistochemical staining was used to detect IA29 on rat uveal vascular endothelium. Results. The ability of IA29 to bind T cell blasts was present to a 1:2000 dilution, and IA29 was readily detectable on uveal vascular endothelium following systemic administration. However, there was no significant difference (p > 0.05) in incidence, time of onset, or severity or histological appearance of EMIU for the rats treated with IA29 when compared with the control rats. Intravital microscopy revealed sticking of leukocytes in the iris vasculature in both groups. Conclusions. We were unable to demonstrate an inhibitory effect of anti-rat ICAM-1 antibody on the outcome of EMIU. Our observations may reflect a redundancy in the adhesion molecule profile responsible for this uveitis.
AB - Purpose. Experimental melanin-induced uveitis (EMIU) is a T cell-mediated rat model of acute anterior uveitis. We investigated the possibility of preventing the inflammation with monoclonal antibody directed against rat intercellular adhesion molecule 1 (ICAM-1). Methods. To induce EMIU, Lewis rats were immunized with bovine ocular melanin extract (250-500 μg). Each day from day 6 post-immunization, rats were injected intraperitoneally with anti-ICAM-1 monoclonal antibody (IA29) or normal mouse serum, and examined with a slit-lamp biomicroscope. On the first day of clinical inflammation, intravital microscopy of iris vasculature was performed on each animal following intraperitoneal injection of rhodamine 6G. At the peak of clinical inflammation, rats were killed, and eyes were examined histologically. Binding potency of IA29 was tested by flow cytometry using concanavalin A-stimulated rat T cells. Immunohistochemical staining was used to detect IA29 on rat uveal vascular endothelium. Results. The ability of IA29 to bind T cell blasts was present to a 1:2000 dilution, and IA29 was readily detectable on uveal vascular endothelium following systemic administration. However, there was no significant difference (p > 0.05) in incidence, time of onset, or severity or histological appearance of EMIU for the rats treated with IA29 when compared with the control rats. Intravital microscopy revealed sticking of leukocytes in the iris vasculature in both groups. Conclusions. We were unable to demonstrate an inhibitory effect of anti-rat ICAM-1 antibody on the outcome of EMIU. Our observations may reflect a redundancy in the adhesion molecule profile responsible for this uveitis.
KW - Anti-ICAM-1 antibody
KW - Cell adhesion molecule
KW - Experimental melanin-induced uveitis
KW - Intravital microscopy
KW - Rat
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U2 - 10.1076/ceyr.21.5.906.5537
DO - 10.1076/ceyr.21.5.906.5537
M3 - Article
C2 - 11262613
AN - SCOPUS:0034465999
SN - 0271-3683
VL - 21
SP - 906
EP - 912
JO - Current Eye Research
JF - Current Eye Research
IS - 5
ER -