Anti-rat ICAM-1 antibody does not influence the course of experimental melanin-induced uveitis

J. R. Smith, L. M. O'Rourke, M. D. Becker, M. Cao, K. A. Williams, Stephen Planck, James (Jim) Rosenbaum

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Purpose. Experimental melanin-induced uveitis (EMIU) is a T cell-mediated rat model of acute anterior uveitis. We investigated the possibility of preventing the inflammation with monoclonal antibody directed against rat intercellular adhesion molecule 1 (ICAM-1). Methods. To induce EMIU, Lewis rats were immunized with bovine ocular melanin extract (250-500 μg). Each day from day 6 post-immunization, rats were injected intraperitoneally with anti-ICAM-1 monoclonal antibody (IA29) or normal mouse serum, and examined with a slit-lamp biomicroscope. On the first day of clinical inflammation, intravital microscopy of iris vasculature was performed on each animal following intraperitoneal injection of rhodamine 6G. At the peak of clinical inflammation, rats were killed, and eyes were examined histologically. Binding potency of IA29 was tested by flow cytometry using concanavalin A-stimulated rat T cells. Immunohistochemical staining was used to detect IA29 on rat uveal vascular endothelium. Results. The ability of IA29 to bind T cell blasts was present to a 1:2000 dilution, and IA29 was readily detectable on uveal vascular endothelium following systemic administration. However, there was no significant difference (p > 0.05) in incidence, time of onset, or severity or histological appearance of EMIU for the rats treated with IA29 when compared with the control rats. Intravital microscopy revealed sticking of leukocytes in the iris vasculature in both groups. Conclusions. We were unable to demonstrate an inhibitory effect of anti-rat ICAM-1 antibody on the outcome of EMIU. Our observations may reflect a redundancy in the adhesion molecule profile responsible for this uveitis.

Original languageEnglish (US)
Pages (from-to)906-912
Number of pages7
JournalCurrent Eye Research
Volume21
Issue number5
DOIs
StatePublished - 2000

Fingerprint

Melanins
Uveitis
Antibodies
Vascular Endothelium
Iris
Inflammation
T-Lymphocytes
Monoclonal Antibodies
Anterior Uveitis
rat ICAM1 protein
Intercellular Adhesion Molecule-1
Concanavalin A
Intraperitoneal Injections
Immunization
Flow Cytometry
Leukocytes
Staining and Labeling
Incidence
Serum

Keywords

  • Anti-ICAM-1 antibody
  • Cell adhesion molecule
  • Experimental melanin-induced uveitis
  • Intravital microscopy
  • Rat

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Anti-rat ICAM-1 antibody does not influence the course of experimental melanin-induced uveitis. / Smith, J. R.; O'Rourke, L. M.; Becker, M. D.; Cao, M.; Williams, K. A.; Planck, Stephen; Rosenbaum, James (Jim).

In: Current Eye Research, Vol. 21, No. 5, 2000, p. 906-912.

Research output: Contribution to journalArticle

Smith, J. R. ; O'Rourke, L. M. ; Becker, M. D. ; Cao, M. ; Williams, K. A. ; Planck, Stephen ; Rosenbaum, James (Jim). / Anti-rat ICAM-1 antibody does not influence the course of experimental melanin-induced uveitis. In: Current Eye Research. 2000 ; Vol. 21, No. 5. pp. 906-912.
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abstract = "Purpose. Experimental melanin-induced uveitis (EMIU) is a T cell-mediated rat model of acute anterior uveitis. We investigated the possibility of preventing the inflammation with monoclonal antibody directed against rat intercellular adhesion molecule 1 (ICAM-1). Methods. To induce EMIU, Lewis rats were immunized with bovine ocular melanin extract (250-500 μg). Each day from day 6 post-immunization, rats were injected intraperitoneally with anti-ICAM-1 monoclonal antibody (IA29) or normal mouse serum, and examined with a slit-lamp biomicroscope. On the first day of clinical inflammation, intravital microscopy of iris vasculature was performed on each animal following intraperitoneal injection of rhodamine 6G. At the peak of clinical inflammation, rats were killed, and eyes were examined histologically. Binding potency of IA29 was tested by flow cytometry using concanavalin A-stimulated rat T cells. Immunohistochemical staining was used to detect IA29 on rat uveal vascular endothelium. Results. The ability of IA29 to bind T cell blasts was present to a 1:2000 dilution, and IA29 was readily detectable on uveal vascular endothelium following systemic administration. However, there was no significant difference (p > 0.05) in incidence, time of onset, or severity or histological appearance of EMIU for the rats treated with IA29 when compared with the control rats. Intravital microscopy revealed sticking of leukocytes in the iris vasculature in both groups. Conclusions. We were unable to demonstrate an inhibitory effect of anti-rat ICAM-1 antibody on the outcome of EMIU. Our observations may reflect a redundancy in the adhesion molecule profile responsible for this uveitis.",
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AU - Smith, J. R.

AU - O'Rourke, L. M.

AU - Becker, M. D.

AU - Cao, M.

AU - Williams, K. A.

AU - Planck, Stephen

AU - Rosenbaum, James (Jim)

PY - 2000

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N2 - Purpose. Experimental melanin-induced uveitis (EMIU) is a T cell-mediated rat model of acute anterior uveitis. We investigated the possibility of preventing the inflammation with monoclonal antibody directed against rat intercellular adhesion molecule 1 (ICAM-1). Methods. To induce EMIU, Lewis rats were immunized with bovine ocular melanin extract (250-500 μg). Each day from day 6 post-immunization, rats were injected intraperitoneally with anti-ICAM-1 monoclonal antibody (IA29) or normal mouse serum, and examined with a slit-lamp biomicroscope. On the first day of clinical inflammation, intravital microscopy of iris vasculature was performed on each animal following intraperitoneal injection of rhodamine 6G. At the peak of clinical inflammation, rats were killed, and eyes were examined histologically. Binding potency of IA29 was tested by flow cytometry using concanavalin A-stimulated rat T cells. Immunohistochemical staining was used to detect IA29 on rat uveal vascular endothelium. Results. The ability of IA29 to bind T cell blasts was present to a 1:2000 dilution, and IA29 was readily detectable on uveal vascular endothelium following systemic administration. However, there was no significant difference (p > 0.05) in incidence, time of onset, or severity or histological appearance of EMIU for the rats treated with IA29 when compared with the control rats. Intravital microscopy revealed sticking of leukocytes in the iris vasculature in both groups. Conclusions. We were unable to demonstrate an inhibitory effect of anti-rat ICAM-1 antibody on the outcome of EMIU. Our observations may reflect a redundancy in the adhesion molecule profile responsible for this uveitis.

AB - Purpose. Experimental melanin-induced uveitis (EMIU) is a T cell-mediated rat model of acute anterior uveitis. We investigated the possibility of preventing the inflammation with monoclonal antibody directed against rat intercellular adhesion molecule 1 (ICAM-1). Methods. To induce EMIU, Lewis rats were immunized with bovine ocular melanin extract (250-500 μg). Each day from day 6 post-immunization, rats were injected intraperitoneally with anti-ICAM-1 monoclonal antibody (IA29) or normal mouse serum, and examined with a slit-lamp biomicroscope. On the first day of clinical inflammation, intravital microscopy of iris vasculature was performed on each animal following intraperitoneal injection of rhodamine 6G. At the peak of clinical inflammation, rats were killed, and eyes were examined histologically. Binding potency of IA29 was tested by flow cytometry using concanavalin A-stimulated rat T cells. Immunohistochemical staining was used to detect IA29 on rat uveal vascular endothelium. Results. The ability of IA29 to bind T cell blasts was present to a 1:2000 dilution, and IA29 was readily detectable on uveal vascular endothelium following systemic administration. However, there was no significant difference (p > 0.05) in incidence, time of onset, or severity or histological appearance of EMIU for the rats treated with IA29 when compared with the control rats. Intravital microscopy revealed sticking of leukocytes in the iris vasculature in both groups. Conclusions. We were unable to demonstrate an inhibitory effect of anti-rat ICAM-1 antibody on the outcome of EMIU. Our observations may reflect a redundancy in the adhesion molecule profile responsible for this uveitis.

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KW - Intravital microscopy

KW - Rat

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