Anti-Hu immunolabeling as an index of neuronal differentiation in human brain tumors

A study of 112 central neuroepithelial neoplasms

Sakir Gultekin, Josep Dalmau, Yvo Graus, Jerome B. Posner, Marc K. Rosenblum

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Anti-Hu is a polyclonal immunoglobulin G associated with a syndrome of paraneoplastic sensory neuropathy/encephalomyelitis that principally afflicts patients with small cell lung carcinoma. Anti-Hu antibodies, which identify a family of RNA-binding proteins that are normally neuron restricted and that appear to be integral to neuronal differentiation and maintenance, selectively label the nuclei (and, less strongly, the cytoplasm) of neurons throughout the human neuraxis. Small cell carcinomas of the lung and many neuroblastomas are also labeled. We screened 112 tumors of central neuroepithelial lineage for immunohistochemical evidence of Hu expression with anti-Hu immunoglobulin G that was purified from patient sera and with a recombinant Fab fragment (Fab GLN 495) selected from a patient-derived combinatorial antibody phage display library using a recombinant Hu protein (HuD). Both antibodies uniformly labeled, in addition to native neurons, the nuclei of central neurocytomas (6 of 6) and the neuronal components of 'classic' (12 of 12) and desmoplastic infantile (2 of 2) gangliogliomas. Of 33 embryonal tumors, 29 were anti-Hu reactive, including 87% of medulloblastomas (26 of 30). Glial neoplasms (n = 59) were anti-Hu negative save for one 'oligodendroglioma' (of 17 oligodendroglial/oligoastrocytic tumors) that may have been an extraventricular neurocytoma. Anti-Hu immunoglobulin G/Fab GLN 495 identifies neoplasms of differentiated neuronal type and embryonal tumors with neuronogenic potential.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalAmerican Journal of Surgical Pathology
Volume22
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Neuroepithelial Neoplasms
Brain Neoplasms
Neurocytoma
Immunoglobulin G
Small Cell Lung Carcinoma
Neoplasms
Neurons
Paraneoplastic Polyneuropathy
Ganglioglioma
Encephalomyelitis
Oligodendroglioma
Immunoglobulin Fab Fragments
Medulloblastoma
RNA-Binding Proteins
Antibodies
Neuroblastoma
Recombinant Proteins
Neuroglia
Bacteriophages
Libraries

Keywords

  • Anti-Hu antineuronal autoantibodies
  • Brain tumors
  • Neuronal neoplasms
  • Paraneoplastic disease
  • Recombinant antibodies

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Anti-Hu immunolabeling as an index of neuronal differentiation in human brain tumors : A study of 112 central neuroepithelial neoplasms. / Gultekin, Sakir; Dalmau, Josep; Graus, Yvo; Posner, Jerome B.; Rosenblum, Marc K.

In: American Journal of Surgical Pathology, Vol. 22, No. 2, 1998, p. 195-200.

Research output: Contribution to journalArticle

Gultekin, Sakir ; Dalmau, Josep ; Graus, Yvo ; Posner, Jerome B. ; Rosenblum, Marc K. / Anti-Hu immunolabeling as an index of neuronal differentiation in human brain tumors : A study of 112 central neuroepithelial neoplasms. In: American Journal of Surgical Pathology. 1998 ; Vol. 22, No. 2. pp. 195-200.
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