TY - JOUR
T1 - Antagonism of ethanol-induced depression of mouse locomotor activity by hyperbaric exposure
AU - Syapin, P. J.
AU - Chen, J.
AU - Finn, D. A.
AU - Alkana, R. L.
N1 - Funding Information:
This work was supported by PHS research grant ROI AA03972 from the Natlona\] Institute on Alcohol Abuse and Alcoholism. We thank Dr. Vlcki Po\]lock for her exce\]lent help with the statistical analysis and Brenda Jones for technical assistance. This work was presented previous\]y in abstract Form (Syapin, et al, Soc. Neurosci. Abst. \]1 143 1985).
PY - 1988
Y1 - 1988
N2 - Previous studies have shown that exposure to hyperbaric helium + oxygen (HEOX) antagonizes the acute depressant effect of hypnotic doses of ethanol on rodent behavior, precipitates and exacerbates withdrawal in ethanol-dependent mice, and attenuates the development of chronic functional ethanol tolerance. The present study extends these investigations to the sub-hypnotic dose range by determining the effect of hyperbaric exposure on ethanol-induced depression of locomotor activity. Male C57BL/6J mice were given two treatments, 2.5 g/kg ethanol and saline, spaced one week apart according to a within subjects, balanced crossover design. Following injection, animals were exposed individually to 1 atmosphere absolute (ATA) air or to 1 ATA or 12 ATA HEOX inside a 15 liter hyperbaric chamber. Chamber temperatures were adjusted to offset ethanol hypothermia and the cooling effect of helium. Locomotor activity was measured continously, beginning 10 min after injection, and recorded at prescribed intervals for 60 min. Multivariate analysis of variance of the measured activity revealed statistically significant differences between groups based on atmospheric condition, treatment, and time after injection. Within group comparisons indicated that ethanol treatment induced a significant reduction in locomotor activity in mice exposed to either 1 ATA air or 1 ATA HEOX. In contrast, ethanol-injected mice exposed to 12 ATA HEOX did not show a significant ethanol-injeced decrease in locomotor activity, indicating antagonism of ethanol's effect. Hyperbaric exposure did not significantly alter blood ethanol concentrations measured 70 min after ethanol injection, thus making a pharmacokinetic explanation for these results unlikely. These findings are consistent with, and extend, previous evidence suggesting that hyperbaric exposure antagonizes molecular actions of ethanol leading to intoxication.
AB - Previous studies have shown that exposure to hyperbaric helium + oxygen (HEOX) antagonizes the acute depressant effect of hypnotic doses of ethanol on rodent behavior, precipitates and exacerbates withdrawal in ethanol-dependent mice, and attenuates the development of chronic functional ethanol tolerance. The present study extends these investigations to the sub-hypnotic dose range by determining the effect of hyperbaric exposure on ethanol-induced depression of locomotor activity. Male C57BL/6J mice were given two treatments, 2.5 g/kg ethanol and saline, spaced one week apart according to a within subjects, balanced crossover design. Following injection, animals were exposed individually to 1 atmosphere absolute (ATA) air or to 1 ATA or 12 ATA HEOX inside a 15 liter hyperbaric chamber. Chamber temperatures were adjusted to offset ethanol hypothermia and the cooling effect of helium. Locomotor activity was measured continously, beginning 10 min after injection, and recorded at prescribed intervals for 60 min. Multivariate analysis of variance of the measured activity revealed statistically significant differences between groups based on atmospheric condition, treatment, and time after injection. Within group comparisons indicated that ethanol treatment induced a significant reduction in locomotor activity in mice exposed to either 1 ATA air or 1 ATA HEOX. In contrast, ethanol-injected mice exposed to 12 ATA HEOX did not show a significant ethanol-injeced decrease in locomotor activity, indicating antagonism of ethanol's effect. Hyperbaric exposure did not significantly alter blood ethanol concentrations measured 70 min after ethanol injection, thus making a pharmacokinetic explanation for these results unlikely. These findings are consistent with, and extend, previous evidence suggesting that hyperbaric exposure antagonizes molecular actions of ethanol leading to intoxication.
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U2 - 10.1016/0024-3205(88)90415-8
DO - 10.1016/0024-3205(88)90415-8
M3 - Article
C2 - 3210903
AN - SCOPUS:0024263707
SN - 0024-3205
VL - 43
SP - 2221
EP - 2229
JO - Life Sciences
JF - Life Sciences
IS - 26
ER -