Angiotensinogen Thr235 variant is associated with abnormal physiologic change of the uterine spiral arteries in first-trimester decidua

Terry Morgan, C. Craven, J. M. Lalouel, K. Ward

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

OBJECTIVE: The angiotensinogen Thr235 polymorphism associated with preeclampsia is tightly linked to a mutation in the angiotensinogen promoter A(-6), which may lead to elevated expression in decidual spiral arteries. We hypothesize that locally elevated angiotensin II levels play a role in failed physiologic change leading to preeclampsia. Our objective was to determine whether spiral artery morphologic characteristics were different in first- trimester decidual samples from women homozygous for the angiotensinogen Thr235 allele and women homozygous for the normal angiotensinogen Met235 allele. STUDY DESIGN: We used quantitative histologic analysis to study 1266 spiral artery cross-sections in decidual samples obtained from normal pregnancies (n = 53) terminated at 8 weeks' gestation. To define vessel characteristics before pregnancy-induced remodeling, we also examined 60 arteries in nonpregnant endometrial control samples (n = 5). We measured the aspect ratio, media area, and external diameter of each cross-section with Image-Pro plus software. Maternal angiotensinogen genotypes were determined by means of mutagenically separated polymerase chain reaction. Average spiral artery morphologic measurements were compared between genotypes with the Student t test. RESULTS: The media area/external diameter ratio was lower in decidual samples than in endometrial samples (P <.0001), consistent with pregnancy-induced physiologic changes. Women homozygous for the angiotensinogen Thr235 allele (n = 11) had a greater area/diameter ratio than did women homozygous for the normal angiotensinogen Met235 allele (n = 11, P <.05). Samples from heterozygous women (n = 31) had intermediate values. CONCLUSION: Our results suggest that the angiotensinogen Thr235 allele predisposes women toward abnormal physiologic change, potentially beginning the cascade of events leading to preeclampsia.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalAmerican Journal of Obstetrics and Gynecology
Volume180
Issue number1 I
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Decidua
Uterine Artery
Angiotensinogen
First Pregnancy Trimester
Arteries
Alleles
Pre-Eclampsia
Pregnancy
Genotype
Angiotensin II
Software
Mothers
Students
Polymerase Chain Reaction
Mutation

Keywords

  • Angiotensinogen Thr235
  • Physiologic Change
  • Preeclampsia

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Angiotensinogen Thr235 variant is associated with abnormal physiologic change of the uterine spiral arteries in first-trimester decidua. / Morgan, Terry; Craven, C.; Lalouel, J. M.; Ward, K.

In: American Journal of Obstetrics and Gynecology, Vol. 180, No. 1 I, 1999, p. 95-102.

Research output: Contribution to journalArticle

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