Angiotensinogen T235 expression is elevated in decidual spiral arteries

Terry Morgan, Catherine Craven, Lesa Nelson, Jean Marc Lalouel, Kenneth Ward

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Preeclampsia is associated with a common molecular variant of angiotensinogen (Met235Thr). This variant is in tight linkage disequilibrium with a mutation in the angiotensinogen promoter, G(-6)A, which leads to elevated expression in vitro. Since angiotensin II levels could play a role in atherotic changes of the uterine spiral arteries associated with preeclampsia, we investigated angiotensinogen expression in the first trimester uterus. We localized angiotensinogen transcription in uterine decidua using in situ reverse transcription PCR. We then compared decidual T235 expression levels to M235 levels in heterozygous women using an allele- specific ligation assay and a single nucleotide primer extension assay. In human decidua, angiotensinogen is expressed only in spiral artery smooth muscle cells. Heterozygous women have significantly elevated expression of the T235 allele compared to the M235 allele (P <0.0001). These observations suggest that elevated expression of the T235 allele in decidual spiral arteries may cause first trimester atherotic changes leading to preeclampsia.

Original languageEnglish (US)
Pages (from-to)1406-1415
Number of pages10
JournalJournal of Clinical Investigation
Volume100
Issue number6
StatePublished - Sep 15 1997
Externally publishedYes

Fingerprint

Angiotensinogen
Arteries
Pre-Eclampsia
Alleles
Decidua
First Pregnancy Trimester
Uterine Artery
Linkage Disequilibrium
Angiotensin II
Reverse Transcription
Uterus
Smooth Muscle Myocytes
Ligation
Nucleotides
Polymerase Chain Reaction
Mutation

Keywords

  • Allele-specific expression
  • In situ RT-PCR
  • Preeclampsia

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Morgan, T., Craven, C., Nelson, L., Lalouel, J. M., & Ward, K. (1997). Angiotensinogen T235 expression is elevated in decidual spiral arteries. Journal of Clinical Investigation, 100(6), 1406-1415.

Angiotensinogen T235 expression is elevated in decidual spiral arteries. / Morgan, Terry; Craven, Catherine; Nelson, Lesa; Lalouel, Jean Marc; Ward, Kenneth.

In: Journal of Clinical Investigation, Vol. 100, No. 6, 15.09.1997, p. 1406-1415.

Research output: Contribution to journalArticle

Morgan, T, Craven, C, Nelson, L, Lalouel, JM & Ward, K 1997, 'Angiotensinogen T235 expression is elevated in decidual spiral arteries', Journal of Clinical Investigation, vol. 100, no. 6, pp. 1406-1415.
Morgan T, Craven C, Nelson L, Lalouel JM, Ward K. Angiotensinogen T235 expression is elevated in decidual spiral arteries. Journal of Clinical Investigation. 1997 Sep 15;100(6):1406-1415.
Morgan, Terry ; Craven, Catherine ; Nelson, Lesa ; Lalouel, Jean Marc ; Ward, Kenneth. / Angiotensinogen T235 expression is elevated in decidual spiral arteries. In: Journal of Clinical Investigation. 1997 ; Vol. 100, No. 6. pp. 1406-1415.
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