Angiotensin II type 1 and 2 receptors in conduit arteries of normal developing microswine

Susan Bagby, Linda S. LeBard, Zaiming Luo, Robert C. Speth, Bryan E. Ogden, Christopher Corless

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective - To identify vascular cells capable of responding to angiotensin II (Ang II) generated in conduit arteries, we examined the Ang II type 1 receptor (AT1R) and Ang II type 2 receptor (AT2R) in the thoracic aorta (TA) and abdominal aorta (AA) and branches in 90-day fetal, 3-week postnatal, and 6-month adult microswine. Methods and Results - By autoradiography (125I-[Sar1Ile8]-Ang II with or without AT1R- or AT2R-selective analogues or 125I-CGP 42112), there were striking rostrocaudal differences in (1) AT2R binding at all ages (prominent in AA wall and branches, sparse in TA wall and branches) and (2) a non-AT2R binding site for CGP 42112 (consistently evident in postnatal TA and branches but absent in AA and branches). Furthermore, patterns of AT2R distribution in infradiaphragmatic arteries were developmentally distinct. In fetal AAs, high-density AT2Rs occupied the inner 60% of the medial-endothelial wall. In postnatal AAs, AT2Rs were sparse in the medial-endothelial wall but prominent in a circumferential smooth muscle α-actin-negative cell layer at the medial-adventitial border, occupying ≈20% to 25% of the AA cross-sectional area. AT1R density in the TA and AA medial- endothelial wall increased with age, whereas AT2R density decreased after birth. Conclusions - A novel AT2R-positive cell layer confined to postnatal infradiaphragmatic arteries physically links adventitial and medial layers, appears optimally positioned to transduce AT2R-dependent functions of local Ang II, and suggests that adventitial Ang II may elicit regionally distinct vascular responses.

Original languageEnglish (US)
Pages (from-to)1113-1121
Number of pages9
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume22
Issue number7
DOIs
StatePublished - 2002

Fingerprint

Angiotensin Type 2 Receptor
Angiotensin Type 1 Receptor
Abdominal Aorta
Thoracic Aorta
Arteries
Angiotensin II
Adventitia
Blood Vessels
Abdominal Wall
Thoracic Wall
Autoradiography
Smooth Muscle
Actins
Binding Sites
Parturition

Keywords

  • CGP 42112
  • Developing aorta
  • Neural AT2R
  • Quantitative autoradiography
  • Vascular renin-angiotensin system

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Angiotensin II type 1 and 2 receptors in conduit arteries of normal developing microswine. / Bagby, Susan; LeBard, Linda S.; Luo, Zaiming; Speth, Robert C.; Ogden, Bryan E.; Corless, Christopher.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 22, No. 7, 2002, p. 1113-1121.

Research output: Contribution to journalArticle

Bagby, Susan ; LeBard, Linda S. ; Luo, Zaiming ; Speth, Robert C. ; Ogden, Bryan E. ; Corless, Christopher. / Angiotensin II type 1 and 2 receptors in conduit arteries of normal developing microswine. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2002 ; Vol. 22, No. 7. pp. 1113-1121.
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abstract = "Objective - To identify vascular cells capable of responding to angiotensin II (Ang II) generated in conduit arteries, we examined the Ang II type 1 receptor (AT1R) and Ang II type 2 receptor (AT2R) in the thoracic aorta (TA) and abdominal aorta (AA) and branches in 90-day fetal, 3-week postnatal, and 6-month adult microswine. Methods and Results - By autoradiography (125I-[Sar1Ile8]-Ang II with or without AT1R- or AT2R-selective analogues or 125I-CGP 42112), there were striking rostrocaudal differences in (1) AT2R binding at all ages (prominent in AA wall and branches, sparse in TA wall and branches) and (2) a non-AT2R binding site for CGP 42112 (consistently evident in postnatal TA and branches but absent in AA and branches). Furthermore, patterns of AT2R distribution in infradiaphragmatic arteries were developmentally distinct. In fetal AAs, high-density AT2Rs occupied the inner 60{\%} of the medial-endothelial wall. In postnatal AAs, AT2Rs were sparse in the medial-endothelial wall but prominent in a circumferential smooth muscle α-actin-negative cell layer at the medial-adventitial border, occupying ≈20{\%} to 25{\%} of the AA cross-sectional area. AT1R density in the TA and AA medial- endothelial wall increased with age, whereas AT2R density decreased after birth. Conclusions - A novel AT2R-positive cell layer confined to postnatal infradiaphragmatic arteries physically links adventitial and medial layers, appears optimally positioned to transduce AT2R-dependent functions of local Ang II, and suggests that adventitial Ang II may elicit regionally distinct vascular responses.",
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T1 - Angiotensin II type 1 and 2 receptors in conduit arteries of normal developing microswine

AU - Bagby, Susan

AU - LeBard, Linda S.

AU - Luo, Zaiming

AU - Speth, Robert C.

AU - Ogden, Bryan E.

AU - Corless, Christopher

PY - 2002

Y1 - 2002

N2 - Objective - To identify vascular cells capable of responding to angiotensin II (Ang II) generated in conduit arteries, we examined the Ang II type 1 receptor (AT1R) and Ang II type 2 receptor (AT2R) in the thoracic aorta (TA) and abdominal aorta (AA) and branches in 90-day fetal, 3-week postnatal, and 6-month adult microswine. Methods and Results - By autoradiography (125I-[Sar1Ile8]-Ang II with or without AT1R- or AT2R-selective analogues or 125I-CGP 42112), there were striking rostrocaudal differences in (1) AT2R binding at all ages (prominent in AA wall and branches, sparse in TA wall and branches) and (2) a non-AT2R binding site for CGP 42112 (consistently evident in postnatal TA and branches but absent in AA and branches). Furthermore, patterns of AT2R distribution in infradiaphragmatic arteries were developmentally distinct. In fetal AAs, high-density AT2Rs occupied the inner 60% of the medial-endothelial wall. In postnatal AAs, AT2Rs were sparse in the medial-endothelial wall but prominent in a circumferential smooth muscle α-actin-negative cell layer at the medial-adventitial border, occupying ≈20% to 25% of the AA cross-sectional area. AT1R density in the TA and AA medial- endothelial wall increased with age, whereas AT2R density decreased after birth. Conclusions - A novel AT2R-positive cell layer confined to postnatal infradiaphragmatic arteries physically links adventitial and medial layers, appears optimally positioned to transduce AT2R-dependent functions of local Ang II, and suggests that adventitial Ang II may elicit regionally distinct vascular responses.

AB - Objective - To identify vascular cells capable of responding to angiotensin II (Ang II) generated in conduit arteries, we examined the Ang II type 1 receptor (AT1R) and Ang II type 2 receptor (AT2R) in the thoracic aorta (TA) and abdominal aorta (AA) and branches in 90-day fetal, 3-week postnatal, and 6-month adult microswine. Methods and Results - By autoradiography (125I-[Sar1Ile8]-Ang II with or without AT1R- or AT2R-selective analogues or 125I-CGP 42112), there were striking rostrocaudal differences in (1) AT2R binding at all ages (prominent in AA wall and branches, sparse in TA wall and branches) and (2) a non-AT2R binding site for CGP 42112 (consistently evident in postnatal TA and branches but absent in AA and branches). Furthermore, patterns of AT2R distribution in infradiaphragmatic arteries were developmentally distinct. In fetal AAs, high-density AT2Rs occupied the inner 60% of the medial-endothelial wall. In postnatal AAs, AT2Rs were sparse in the medial-endothelial wall but prominent in a circumferential smooth muscle α-actin-negative cell layer at the medial-adventitial border, occupying ≈20% to 25% of the AA cross-sectional area. AT1R density in the TA and AA medial- endothelial wall increased with age, whereas AT2R density decreased after birth. Conclusions - A novel AT2R-positive cell layer confined to postnatal infradiaphragmatic arteries physically links adventitial and medial layers, appears optimally positioned to transduce AT2R-dependent functions of local Ang II, and suggests that adventitial Ang II may elicit regionally distinct vascular responses.

KW - CGP 42112

KW - Developing aorta

KW - Neural AT2R

KW - Quantitative autoradiography

KW - Vascular renin-angiotensin system

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