TY - JOUR
T1 - Androgen receptor gene expression in the rat central nervous system
T2 - Evidence for two mRNA transcripts
AU - McLachlan, Robert I.
AU - Tempel, Bruce L.
AU - Miller, Margaret A.
AU - Bicknell, James N.
AU - Bremner, William J.
AU - Dorsa, Daniel M.
N1 - Funding Information:
This research was supported by the Department and National Institutes of Health Grant NS20311 Population Research Center of the University HD12629). Robert McLachlan is a Neil Hamilton Australian National Health and Medical Research of Veterans Affairs and by the NICHD of Washington (P50 Fairley Fellow of the Council.
PY - 1991/4
Y1 - 1991/4
N2 - Androgen receptor (AR) gene expression in the central nervous system (CNS) and peripheral tissues of male rats was examined using cDNA probes to measure AR mRNA by RNA (Northern) blot analysis and by in situ hybridization. Using a probe from the 5′ untranslated region of the rat cDNA (AR-1), a single mRNA species of approximately 11 kb was seen in Northern blots of poly(A)+ RNA from reproductive tissues, kidney, liver, and muscle. Using a probe from the 5′ end of the coding region (AR-2), in addition to the 11-kb band, a novel transcript was seen in whole brain at about 9.3 kb. In poly(A)+ RNA from dissected brain regions, the 9.3-kb transcript was predominant in the cortex, cerebellum, and brain stem, while in the hippocampus, both transcripts were expressed to a similar degree. AR mRNA levels increased two- to threefold in the prostate on Days 1 and 3 following castration but no significant change was seen in either CNS transcript in whole brain or cortex. Specific in situ hybridization of an 35S-labeled AR-2 riboprobe was observed in brain regions known to bind radiolabeled androgens. We conclude that two AR RNA species exist in the adult male rat which differ in their 5′ untranslated region and that the relative proportion of the two species varies between brain regions. In contrast to observations in the prostate, AR gene expression in the cerebral cortex is not regulated in the short term by androgen withdrawal.
AB - Androgen receptor (AR) gene expression in the central nervous system (CNS) and peripheral tissues of male rats was examined using cDNA probes to measure AR mRNA by RNA (Northern) blot analysis and by in situ hybridization. Using a probe from the 5′ untranslated region of the rat cDNA (AR-1), a single mRNA species of approximately 11 kb was seen in Northern blots of poly(A)+ RNA from reproductive tissues, kidney, liver, and muscle. Using a probe from the 5′ end of the coding region (AR-2), in addition to the 11-kb band, a novel transcript was seen in whole brain at about 9.3 kb. In poly(A)+ RNA from dissected brain regions, the 9.3-kb transcript was predominant in the cortex, cerebellum, and brain stem, while in the hippocampus, both transcripts were expressed to a similar degree. AR mRNA levels increased two- to threefold in the prostate on Days 1 and 3 following castration but no significant change was seen in either CNS transcript in whole brain or cortex. Specific in situ hybridization of an 35S-labeled AR-2 riboprobe was observed in brain regions known to bind radiolabeled androgens. We conclude that two AR RNA species exist in the adult male rat which differ in their 5′ untranslated region and that the relative proportion of the two species varies between brain regions. In contrast to observations in the prostate, AR gene expression in the cerebral cortex is not regulated in the short term by androgen withdrawal.
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U2 - 10.1016/1044-7431(91)90003-7
DO - 10.1016/1044-7431(91)90003-7
M3 - Article
AN - SCOPUS:0000822549
SN - 1044-7431
VL - 2
SP - 117
EP - 122
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 2
ER -