Androgen receptor gene expression in the rat central nervous system: Evidence for two mRNA transcripts

Robert I. McLachlan, Bruce L. Tempel, Margaret A. Miller, James N. Bicknell, William J. Bremner, Daniel Dorsa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Androgen receptor (AR) gene expression in the central nervous system (CNS) and peripheral tissues of male rats was examined using cDNA probes to measure AR mRNA by RNA (Northern) blot analysis and by in situ hybridization. Using a probe from the 5′ untranslated region of the rat cDNA (AR-1), a single mRNA species of approximately 11 kb was seen in Northern blots of poly(A)+ RNA from reproductive tissues, kidney, liver, and muscle. Using a probe from the 5′ end of the coding region (AR-2), in addition to the 11-kb band, a novel transcript was seen in whole brain at about 9.3 kb. In poly(A)+ RNA from dissected brain regions, the 9.3-kb transcript was predominant in the cortex, cerebellum, and brain stem, while in the hippocampus, both transcripts were expressed to a similar degree. AR mRNA levels increased two- to threefold in the prostate on Days 1 and 3 following castration but no significant change was seen in either CNS transcript in whole brain or cortex. Specific in situ hybridization of an 35S-labeled AR-2 riboprobe was observed in brain regions known to bind radiolabeled androgens. We conclude that two AR RNA species exist in the adult male rat which differ in their 5′ untranslated region and that the relative proportion of the two species varies between brain regions. In contrast to observations in the prostate, AR gene expression in the cerebral cortex is not regulated in the short term by androgen withdrawal.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalMolecular and Cellular Neuroscience
Volume2
Issue number2
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Androgen Receptors
Central Nervous System
Gene Expression
Messenger RNA
Brain
5' Untranslated Regions
Northern Blotting
Androgens
In Situ Hybridization
Prostate
Complementary DNA
RNA
Castration
Cerebral Cortex
Cerebellum
Brain Stem
Hippocampus
Kidney
Muscles
Liver

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Androgen receptor gene expression in the rat central nervous system : Evidence for two mRNA transcripts. / McLachlan, Robert I.; Tempel, Bruce L.; Miller, Margaret A.; Bicknell, James N.; Bremner, William J.; Dorsa, Daniel.

In: Molecular and Cellular Neuroscience, Vol. 2, No. 2, 1991, p. 117-122.

Research output: Contribution to journalArticle

McLachlan, Robert I. ; Tempel, Bruce L. ; Miller, Margaret A. ; Bicknell, James N. ; Bremner, William J. ; Dorsa, Daniel. / Androgen receptor gene expression in the rat central nervous system : Evidence for two mRNA transcripts. In: Molecular and Cellular Neuroscience. 1991 ; Vol. 2, No. 2. pp. 117-122.
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