Androgen receptor characteristics in skin fibroblasts from hirsute women

C. Eil, G. B. Cutler, Donald (Lynn) Loriaux

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Hormonal measurements in some women with hirsutism often reveal little or no elevation in androgen levels to explain the disorder. Thus, it has been postulated that increased sensitivity of the hair follicle to androgen may contribute to the development of hirsutism in such patients. We, therefore, sought androgen receptor abnormalities in skin fibroblasts cultured from 10 hirsute women (ages 17-43) and normal or mildly elevated plasma testosterone levels (28-82 ng/dl). Androgen receptor content (R(O)) and binding affinity (K(d)) in cultured pubic skin fibroblasts were measured using a dispersed, whole cell assay. Ten such cell lines from these women were compared with 19 pubic skin cell lines from 9 normal volunteers (6 males and 3 females) and from 10 other subjects (males with gynecomastia or hypospadias). There was no statistically significant difference in the mean androgen receptor content (11,600 ± 2700 (SE) sites/cell fibroblasts vs 7900 ± 700 sites/cell or binding affinity (2.0 ± 0.3 (SE) x 10-9 M vs 1.5 ± 0.2 x 10-9 M, respectively) between the patients' fibroblasts and those of the controls. We conclude that hirsutism cannot be explained by abnromalities in fibroblast androgen receptor number or affinity. These observations do not exclude the possibility that other mechanisms might lead to increased peripheral androgen sensitivity in such patients.

Original languageEnglish (US)
Pages (from-to)62-65
Number of pages4
JournalJournal of Investigative Dermatology
Volume84
Issue number1
StatePublished - 1985
Externally publishedYes

Fingerprint

Androgen Receptors
Fibroblasts
Skin
Hirsutism
Androgens
Skin Abnormalities
Cells
Gynecomastia
Cell Line
Hypospadias
Hair Follicle
Testosterone
Assays
Healthy Volunteers
Plasmas

ASJC Scopus subject areas

  • Dermatology

Cite this

Androgen receptor characteristics in skin fibroblasts from hirsute women. / Eil, C.; Cutler, G. B.; Loriaux, Donald (Lynn).

In: Journal of Investigative Dermatology, Vol. 84, No. 1, 1985, p. 62-65.

Research output: Contribution to journalArticle

@article{e80f47d208404ab2b94a84c3b25752f6,
title = "Androgen receptor characteristics in skin fibroblasts from hirsute women",
abstract = "Hormonal measurements in some women with hirsutism often reveal little or no elevation in androgen levels to explain the disorder. Thus, it has been postulated that increased sensitivity of the hair follicle to androgen may contribute to the development of hirsutism in such patients. We, therefore, sought androgen receptor abnormalities in skin fibroblasts cultured from 10 hirsute women (ages 17-43) and normal or mildly elevated plasma testosterone levels (28-82 ng/dl). Androgen receptor content (R(O)) and binding affinity (K(d)) in cultured pubic skin fibroblasts were measured using a dispersed, whole cell assay. Ten such cell lines from these women were compared with 19 pubic skin cell lines from 9 normal volunteers (6 males and 3 females) and from 10 other subjects (males with gynecomastia or hypospadias). There was no statistically significant difference in the mean androgen receptor content (11,600 ± 2700 (SE) sites/cell fibroblasts vs 7900 ± 700 sites/cell or binding affinity (2.0 ± 0.3 (SE) x 10-9 M vs 1.5 ± 0.2 x 10-9 M, respectively) between the patients' fibroblasts and those of the controls. We conclude that hirsutism cannot be explained by abnromalities in fibroblast androgen receptor number or affinity. These observations do not exclude the possibility that other mechanisms might lead to increased peripheral androgen sensitivity in such patients.",
author = "C. Eil and Cutler, {G. B.} and Loriaux, {Donald (Lynn)}",
year = "1985",
language = "English (US)",
volume = "84",
pages = "62--65",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Androgen receptor characteristics in skin fibroblasts from hirsute women

AU - Eil, C.

AU - Cutler, G. B.

AU - Loriaux, Donald (Lynn)

PY - 1985

Y1 - 1985

N2 - Hormonal measurements in some women with hirsutism often reveal little or no elevation in androgen levels to explain the disorder. Thus, it has been postulated that increased sensitivity of the hair follicle to androgen may contribute to the development of hirsutism in such patients. We, therefore, sought androgen receptor abnormalities in skin fibroblasts cultured from 10 hirsute women (ages 17-43) and normal or mildly elevated plasma testosterone levels (28-82 ng/dl). Androgen receptor content (R(O)) and binding affinity (K(d)) in cultured pubic skin fibroblasts were measured using a dispersed, whole cell assay. Ten such cell lines from these women were compared with 19 pubic skin cell lines from 9 normal volunteers (6 males and 3 females) and from 10 other subjects (males with gynecomastia or hypospadias). There was no statistically significant difference in the mean androgen receptor content (11,600 ± 2700 (SE) sites/cell fibroblasts vs 7900 ± 700 sites/cell or binding affinity (2.0 ± 0.3 (SE) x 10-9 M vs 1.5 ± 0.2 x 10-9 M, respectively) between the patients' fibroblasts and those of the controls. We conclude that hirsutism cannot be explained by abnromalities in fibroblast androgen receptor number or affinity. These observations do not exclude the possibility that other mechanisms might lead to increased peripheral androgen sensitivity in such patients.

AB - Hormonal measurements in some women with hirsutism often reveal little or no elevation in androgen levels to explain the disorder. Thus, it has been postulated that increased sensitivity of the hair follicle to androgen may contribute to the development of hirsutism in such patients. We, therefore, sought androgen receptor abnormalities in skin fibroblasts cultured from 10 hirsute women (ages 17-43) and normal or mildly elevated plasma testosterone levels (28-82 ng/dl). Androgen receptor content (R(O)) and binding affinity (K(d)) in cultured pubic skin fibroblasts were measured using a dispersed, whole cell assay. Ten such cell lines from these women were compared with 19 pubic skin cell lines from 9 normal volunteers (6 males and 3 females) and from 10 other subjects (males with gynecomastia or hypospadias). There was no statistically significant difference in the mean androgen receptor content (11,600 ± 2700 (SE) sites/cell fibroblasts vs 7900 ± 700 sites/cell or binding affinity (2.0 ± 0.3 (SE) x 10-9 M vs 1.5 ± 0.2 x 10-9 M, respectively) between the patients' fibroblasts and those of the controls. We conclude that hirsutism cannot be explained by abnromalities in fibroblast androgen receptor number or affinity. These observations do not exclude the possibility that other mechanisms might lead to increased peripheral androgen sensitivity in such patients.

UR - http://www.scopus.com/inward/record.url?scp=0021930871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021930871&partnerID=8YFLogxK

M3 - Article

C2 - 3965579

AN - SCOPUS:0021930871

VL - 84

SP - 62

EP - 65

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 1

ER -