Androgen binding proteins of testis, epididymis, and plasma in man and monkey

R. A. Vigersky, Donald (Lynn) Loriaux, S. S. Howards, G. B. Hodgen, M. B. Lipsett, A. Chrambach

Research output: Contribution to journalArticle

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Abstract

Androgen binding protein (ABP) has been found in the cytosol of testicular and epididymal homogenates of several sub primate species. In those species which had the plasma androgen binding protein, testosterone estradiol binding globulin (TeBG), ABP and TeBG were found to be physically similar. We investigated the possibility that ABP might exist in monkey and man using the cytosol of testicular and epididymal homogenates and aspirates obtained by direct micropuncture of the rete testis. In polyacrylamide gel electrophoresis, pH 7.8, testicular and epididymal cytosols of monkey and man were found to contain several binding proteins of different size and net charge that bind dihydrotestosterone. These binding proteins were either indistinguishable from TeBG or could be related to TeBG as size and/or charge isomers. No ABP was detectable in up to 200 μl of monkey rete testis fluid obtained by direct micropuncture, though ABP is detectable in as little as 5 μl of rat rete testis fluid. The data suggest that the ABPs detected in the testicular and epididymal cytosols in monkey and man represent isomeric forms of plasma TeBG, and their presence in testicular cytosol most likely derives from blood contamination.

Original languageEnglish (US)
Pages (from-to)1061-1068
Number of pages8
JournalJournal of Clinical Investigation
Volume58
Issue number5
StatePublished - 1976
Externally publishedYes

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Androgen-Binding Protein
Epididymis
Sex Hormone-Binding Globulin
Haplorhini
Testis
Cytosol
Rete Testis
Punctures
Carrier Proteins
Dihydrotestosterone
Primates
Polyacrylamide Gel Electrophoresis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Vigersky, R. A., Loriaux, D. L., Howards, S. S., Hodgen, G. B., Lipsett, M. B., & Chrambach, A. (1976). Androgen binding proteins of testis, epididymis, and plasma in man and monkey. Journal of Clinical Investigation, 58(5), 1061-1068.

Androgen binding proteins of testis, epididymis, and plasma in man and monkey. / Vigersky, R. A.; Loriaux, Donald (Lynn); Howards, S. S.; Hodgen, G. B.; Lipsett, M. B.; Chrambach, A.

In: Journal of Clinical Investigation, Vol. 58, No. 5, 1976, p. 1061-1068.

Research output: Contribution to journalArticle

Vigersky, RA, Loriaux, DL, Howards, SS, Hodgen, GB, Lipsett, MB & Chrambach, A 1976, 'Androgen binding proteins of testis, epididymis, and plasma in man and monkey', Journal of Clinical Investigation, vol. 58, no. 5, pp. 1061-1068.
Vigersky RA, Loriaux DL, Howards SS, Hodgen GB, Lipsett MB, Chrambach A. Androgen binding proteins of testis, epididymis, and plasma in man and monkey. Journal of Clinical Investigation. 1976;58(5):1061-1068.
Vigersky, R. A. ; Loriaux, Donald (Lynn) ; Howards, S. S. ; Hodgen, G. B. ; Lipsett, M. B. ; Chrambach, A. / Androgen binding proteins of testis, epididymis, and plasma in man and monkey. In: Journal of Clinical Investigation. 1976 ; Vol. 58, No. 5. pp. 1061-1068.
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