Analysis of viral and cellular microRNAs in EBV-infected cells

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

MicroRNAs are small, noncoding RNAs that posttranscriptionally regulate gene expression. The discovery of this relatively new mode of gene regulation as well as studies showing the prognostic value of viral and cellular miRNAs as biomarkers, such as in cancer progression, has stimulated the development of many methods to characterize miRNAs. EBV encodes 25 viral precursor microRNAs within its genome that are expressed during lytic and latent infection. In addition to viral miRNAs, EBV infection induces the expression of specific cellular oncogenic miRNAs, such as miR-155, miR-146a, miR-21, and others, that can contribute to the persistence of latently infected cells. This chapter describes several current techniques used to identify and detect the expression of viral and cellular miRNAs in EBV-infected cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages133-146
Number of pages14
Volume1532
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1532
ISSN (Print)10643745

Keywords

  • BART
  • BHRF1
  • Deep sequencing
  • Luciferase assay
  • MicroRNA
  • Primer extension
  • RIP-seq
  • RISC
  • RNA isolation
  • Stem-loop qRT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Skalsky, R. (2017). Analysis of viral and cellular microRNAs in EBV-infected cells. In Methods in Molecular Biology (Vol. 1532, pp. 133-146). (Methods in Molecular Biology; Vol. 1532). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6655-4_9