Analysis of Tn3 sequences required for transposition and immunity

Huang Chang-Jen, Fred Heffron, Twu -Shin, Robert H. Schloemer, Lee Chao-Hung

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    25 Scopus citations

    Abstract

    Tn3 is a 5-kb transposon (Tn) with 38-bp inverted terminal repeats (ITR). The two 38-bp terminal sequences are required in cis for TnJ transposition. In this study, the role of the ITR in Tn3 transposition has been further dissected by the use of various mini-Tn3 Tn's. The transposition frequency of these mini-Tn's demonstrate that (1) Tn3 contains no sequence other than the ITR sequences that are necessary for the first step in transposition; (2) the two terminal repeats must be oriented as ITR for transposition to occur; (3) the outside 34 bp of the ITR are required for transposition; and (4) reducing the distance between the terminal sequences does not affect transposition frequency. Moreover, mutant copies of the ITR sequences that cannot function in transposition do not confer transposition immunity.

    Original languageEnglish (US)
    Pages (from-to)23-31
    Number of pages9
    JournalGene
    Volume41
    Issue number1
    DOIs
    StatePublished - 1986

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    Keywords

    • Recombinant DNA
    • inverted terminal repeat
    • minitransposon

    ASJC Scopus subject areas

    • Genetics

    Cite this

    Chang-Jen, H., Heffron, F., -Shin, T., Schloemer, R. H., & Chao-Hung, L. (1986). Analysis of Tn3 sequences required for transposition and immunity. Gene, 41(1), 23-31. https://doi.org/10.1016/0378-1119(86)90263-5