Analysis of the sequence and embryonic expression of chicken neurofibromin mRNA

Gwen L. Schafer, Gary Ciment, Kate M. Stocker, Lawrence Baizer

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Neurofibromatosis type 1 (NF1) is a common inherited disorder that primarily affects tissues derived from the neural crest. Recent identification and characterization of the human NF1 gene has revealed that it encodes a protein (now called neurofibromin) that is similar in sequence to the ras-GTPase activator protein (or ras-GAP), suggesting that neurofibromin may be a component of cellular signal transduction pathways regulating cellular proliferation and/or differentiation. To initiate investigations on the role of the NF1 gene product in embryonic development, we have isolated a partial cDNA for chicken neurofibromin. Sequence analysis reveals that the predicted amino acid sequence is highly conserved between chick and human. The chicken cDNA hybridizes to a 12.5-kb transcript on RNA blots, a mol wt similar to that reported for the human and murine mRNAs. Ribonuclease protection assays indicate that NF1 mRNA is expressed in a variety of tissues in the chick embryo; this is confirmed by in situ hybridization analysis. NF1 mRNA expression is detectable as early as embryonic stage 18 in the neural plate. This pattern of expression may suggest a role for neurofibromin during normal development, including that of the nervous system.

Original languageEnglish (US)
Pages (from-to)267-278
Number of pages12
JournalMolecular and Chemical Neuropathology
Volume18
Issue number3
DOIs
StatePublished - Apr 1993

Fingerprint

Neurofibromin 1
Neurofibromatosis 1
Sequence Analysis
Chickens
Messenger RNA
GTP Phosphohydrolase Activators
Complementary DNA
Neurofibromatosis 1 Genes
ras GTPase-Activating Proteins
Neural Plate
ras Proteins
Forensic Anthropology
Neural Crest
Chick Embryo
Ribonucleases
Nervous System
Embryonic Development
In Situ Hybridization
Amino Acid Sequence
Signal Transduction

Keywords

  • chicken embryogenesis
  • in situ hybridization
  • Neurofibromatosis
  • RNA blot
  • RNase protection analysis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Molecular Biology

Cite this

Analysis of the sequence and embryonic expression of chicken neurofibromin mRNA. / Schafer, Gwen L.; Ciment, Gary; Stocker, Kate M.; Baizer, Lawrence.

In: Molecular and Chemical Neuropathology, Vol. 18, No. 3, 04.1993, p. 267-278.

Research output: Contribution to journalArticle

Schafer, Gwen L. ; Ciment, Gary ; Stocker, Kate M. ; Baizer, Lawrence. / Analysis of the sequence and embryonic expression of chicken neurofibromin mRNA. In: Molecular and Chemical Neuropathology. 1993 ; Vol. 18, No. 3. pp. 267-278.
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