Analysis of the organization and nucleotide sequence of the chromosomal gene for the β-subunit of rat thyrotropin

M. L. Croyle, A. Bhattacharya, D. F. Gordon, Richard Maurer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The gene for the β-subunit of rat thyrotropin thas been isolated from a library of rat DNA fragments cloned in bacteriophage λ. The complete nucleotide sequence of the gene has been determined including a portion of 5'-and 3'-flanking regions. The rat TSH-β gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3' poly(A) tract. The first exon which represents only 5' untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalDNA
Volume5
Issue number4
StatePublished - 1986
Externally publishedYes

Fingerprint

Thyrotropin
Rats
Nucleotides
Genes
Exons
Introns
3' Flanking Region
Messenger RNA
Poly A
5' Flanking Region
Southern Blotting
Gene Library
Bacteriophages
Cloning
DNA
Organism Cloning
Genome

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Analysis of the organization and nucleotide sequence of the chromosomal gene for the β-subunit of rat thyrotropin. / Croyle, M. L.; Bhattacharya, A.; Gordon, D. F.; Maurer, Richard.

In: DNA, Vol. 5, No. 4, 1986, p. 299-304.

Research output: Contribution to journalArticle

Croyle, M. L. ; Bhattacharya, A. ; Gordon, D. F. ; Maurer, Richard. / Analysis of the organization and nucleotide sequence of the chromosomal gene for the β-subunit of rat thyrotropin. In: DNA. 1986 ; Vol. 5, No. 4. pp. 299-304.
@article{e4253dd2a74e48a6bc7c0166e2f09192,
title = "Analysis of the organization and nucleotide sequence of the chromosomal gene for the β-subunit of rat thyrotropin",
abstract = "The gene for the β-subunit of rat thyrotropin thas been isolated from a library of rat DNA fragments cloned in bacteriophage λ. The complete nucleotide sequence of the gene has been determined including a portion of 5'-and 3'-flanking regions. The rat TSH-β gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3' poly(A) tract. The first exon which represents only 5' untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process.",
author = "Croyle, {M. L.} and A. Bhattacharya and Gordon, {D. F.} and Richard Maurer",
year = "1986",
language = "English (US)",
volume = "5",
pages = "299--304",
journal = "DNA and Cell Biology",
issn = "1044-5498",
publisher = "Mary Ann Liebert Inc.",
number = "4",

}

TY - JOUR

T1 - Analysis of the organization and nucleotide sequence of the chromosomal gene for the β-subunit of rat thyrotropin

AU - Croyle, M. L.

AU - Bhattacharya, A.

AU - Gordon, D. F.

AU - Maurer, Richard

PY - 1986

Y1 - 1986

N2 - The gene for the β-subunit of rat thyrotropin thas been isolated from a library of rat DNA fragments cloned in bacteriophage λ. The complete nucleotide sequence of the gene has been determined including a portion of 5'-and 3'-flanking regions. The rat TSH-β gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3' poly(A) tract. The first exon which represents only 5' untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process.

AB - The gene for the β-subunit of rat thyrotropin thas been isolated from a library of rat DNA fragments cloned in bacteriophage λ. The complete nucleotide sequence of the gene has been determined including a portion of 5'-and 3'-flanking regions. The rat TSH-β gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3' poly(A) tract. The first exon which represents only 5' untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process.

UR - http://www.scopus.com/inward/record.url?scp=0022498327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022498327&partnerID=8YFLogxK

M3 - Article

C2 - 3017658

AN - SCOPUS:0022498327

VL - 5

SP - 299

EP - 304

JO - DNA and Cell Biology

JF - DNA and Cell Biology

SN - 1044-5498

IS - 4

ER -