Analysis of the human type I insulin-like growth factor receptor promoter region

David W. Cooke, Laura A. Bankert, Charles Roberts, Derek LeRoith, Samuel J. Casella

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

We isolated genomic fragments containing the 5′ region of the human type I insulin-like growth factor receptor gene. A unique transcription start site was identified, defining a 1038 bp 5′-untranslated region. No TATA or CCAAT elements were identified in the proximal 480 nucleotides of 5′-flanking region. The region surrounding the transcription start site was similar to a recently described "initiator" sequence. The 5′-flanking and 5′-untranslated regions were highly GC-rich, with numerous potential Sp1 binding sites. A potential AP-2 binding site was identified in the 5′-flanking region and a potential thyroid response element was identified in the 5′-untranslated region. The 5′ region of the human gene was very similar to that of the rat gene, with conservation of many of the potential regulatory elements.

Original languageEnglish (US)
Pages (from-to)1113-1120
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume177
Issue number3
DOIs
StatePublished - Jun 28 1991
Externally publishedYes

Fingerprint

IGF Type 1 Receptor
5' Untranslated Regions
Genetic Promoter Regions
Genes
5' Flanking Region
Transcription Initiation Site
Binding Sites
Response Elements
Rats
Conservation
Thyroid Gland
Nucleotides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Analysis of the human type I insulin-like growth factor receptor promoter region. / Cooke, David W.; Bankert, Laura A.; Roberts, Charles; LeRoith, Derek; Casella, Samuel J.

In: Biochemical and Biophysical Research Communications, Vol. 177, No. 3, 28.06.1991, p. 1113-1120.

Research output: Contribution to journalArticle

Cooke, David W. ; Bankert, Laura A. ; Roberts, Charles ; LeRoith, Derek ; Casella, Samuel J. / Analysis of the human type I insulin-like growth factor receptor promoter region. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 177, No. 3. pp. 1113-1120.
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