Analysis of the human type I insulin-like growth factor receptor promoter region

David W. Cooke, Laura A. Bankert, Charles T. Roberts, Derek LeRoith, Samuel J. Casella

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    Abstract

    We isolated genomic fragments containing the 5′ region of the human type I insulin-like growth factor receptor gene. A unique transcription start site was identified, defining a 1038 bp 5′-untranslated region. No TATA or CCAAT elements were identified in the proximal 480 nucleotides of 5′-flanking region. The region surrounding the transcription start site was similar to a recently described "initiator" sequence. The 5′-flanking and 5′-untranslated regions were highly GC-rich, with numerous potential Sp1 binding sites. A potential AP-2 binding site was identified in the 5′-flanking region and a potential thyroid response element was identified in the 5′-untranslated region. The 5′ region of the human gene was very similar to that of the rat gene, with conservation of many of the potential regulatory elements.

    Original languageEnglish (US)
    Pages (from-to)1113-1120
    Number of pages8
    JournalBiochemical and Biophysical Research Communications
    Volume177
    Issue number3
    DOIs
    StatePublished - Jun 28 1991

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    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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