Analysis of the cross-reactive anti-gp120 antibody population in human immunodeficiency virus-infected asymptomatic individuals

Kandasamy Hariharan, Peter L. Nara, Vincent M. Caralli, Frank L. Norton, Nancy Haigwood, Chang Yuil Kang

Research output: Contribution to journalArticle

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Abstract

This study was undertaken to analyze the specificity and neutralizing properties of cross-reactive anti-gp120 antibodies (Abs) in the sera of two human immunodeficiency virus (HIV)-infected asymptomatic individuals. Two panels of murine monoclonal anti-idiotype Abs (anti-id MAbs) were established against cross-reactive polyclonal anti-gp120 Abs purified from HIV+ sera by sequential affinity chromatography using gp120SF2- and gp120IIIB-Sepharose columns. These panels of anti-id MAbs were then used to affinity purify idiotype-positive (Id+) anti-gp120 Abs from HIV+ sera. The recovery of each of these Id+ Abs by purification indicated that several idiotypically distinct cross-reactive anti-gp120 Abs are present in sera over a wide range of concentrations. Immunological and biological studies showed that although all of the Id+ Abs were reactive against gp120SF2 and gp120IIIB, they exhibited unique epitope specificities and distinct neutralizing activities. Most of the Id+ Abs were directed against epitopes in the CD4 attachment site (CD4 site epitopes) of gp120 and exhibited a spectrum of broadly neutralizing activities. On the other hand, a minor population of Id+ Abs showed specificity for the V3 region of gp120 and exhibited limited cross-neutralizing activities. Together, these studies indicate that the CD4 site epitope-specific Abs are heterogeneous with respect to their clonality, neutralizing activity, and concentration in sera. This heterogeneity suggests that anti-gp120 Abs to the CD4 attachment site are developed in response to multiple overlapping epitopes present on the original virus isolate and/or epitopes on mutated variants which emerged over time.

Original languageEnglish (US)
Pages (from-to)953-960
Number of pages8
JournalJournal of Virology
Volume67
Issue number2
StatePublished - Feb 1993
Externally publishedYes

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Human immunodeficiency virus
Epitopes
Anti-Idiotypic Antibodies
HIV
antibodies
epitopes
neutralization
Population
Serum
Antibodies
Antibody Specificity
Affinity Chromatography
Sepharose
Monoclonal Antibodies
Viruses
affinity chromatography
agarose
viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the cross-reactive anti-gp120 antibody population in human immunodeficiency virus-infected asymptomatic individuals. / Hariharan, Kandasamy; Nara, Peter L.; Caralli, Vincent M.; Norton, Frank L.; Haigwood, Nancy; Kang, Chang Yuil.

In: Journal of Virology, Vol. 67, No. 2, 02.1993, p. 953-960.

Research output: Contribution to journalArticle

Hariharan, Kandasamy ; Nara, Peter L. ; Caralli, Vincent M. ; Norton, Frank L. ; Haigwood, Nancy ; Kang, Chang Yuil. / Analysis of the cross-reactive anti-gp120 antibody population in human immunodeficiency virus-infected asymptomatic individuals. In: Journal of Virology. 1993 ; Vol. 67, No. 2. pp. 953-960.
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