Analysis of the assembly function of the human immunodeficiency virus type 1 gag protein nucleocapsid domain

Yaqiang Zhang, Haoyu Qian, Zachary Love, Eric Barklis

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Previous studies have shown that in addition to its function in specific RNA encapsidation, the human immunodeficiency virus type 1 (HIV-1) nucleocapsid (NC) is required for efficient virus particle assembly. However, the mechanism by which NC facilitates the assembly process is not clearly established. Formally, NC could act by constraining the Pr55(gag) polyprotein into an assembly-competent conformation or by masking residues which block the assembly process. Alternatively, the capacity of NC to bind RNA or make interprotein contacts might affect particle assembly. To examine its role in the assembly process, we replaced the NC domain in Pr55(gag) with polypeptide domains of known function, and the chimeric proteins were analyzed for their abilities to direct the release of virus-like particles. Our results indicate that NC does not mask inhibitory domains and does not act passively, by simply providing a stable folded monomeric structure. However, replacement of NC by polypeptides which form interprotein contacts permitted efficient virus particle assembly and release, even when RNA was not detected in the particles. These results suggest that formation of interprotein contacts by NC is essential to the normal HIV-1 assembly process.

Original languageEnglish (US)
Pages (from-to)1782-1789
Number of pages8
JournalJournal of Virology
Volume72
Issue number3
StatePublished - Mar 1998

Fingerprint

Human Immunodeficiency Virus gag Gene Products
nucleocapsid proteins
Nucleocapsid
nucleocapsid
Human immunodeficiency virus 1
HIV-1
Virus Assembly
Virion
RNA
virion
polypeptides
recombinant fusion proteins
Virus Release
gag Gene Products
Peptides
virus-like particles
Protein Domains
Masks

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the assembly function of the human immunodeficiency virus type 1 gag protein nucleocapsid domain. / Zhang, Yaqiang; Qian, Haoyu; Love, Zachary; Barklis, Eric.

In: Journal of Virology, Vol. 72, No. 3, 03.1998, p. 1782-1789.

Research output: Contribution to journalArticle

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