Analysis of rhesus rhadinovirus microRNAs expressed in virus-induced tumors from infected rhesus macaques

Jennifer L. Umbach, Lisa I. Strelow, Scott W. Wong, Bryan R. Cullen

Research output: Contribution to journalArticle

33 Scopus citations


Rhesus rhadinovirus (RRV), a primate γ-herpesvirus related to human Kaposi's sarcoma-associated herpesvirus (KSHV), causes a similar pattern of pathogenesis. Previously, RRV was shown to express 7 pre-microRNAs (pre-miRNAs) in latently infected cells. Using deep sequencing, we analyzed the pattern of small RNA expression in vivo using latently RRV-infected B-cell lymphoma and retroperitoneal fibromatosis tissues. We identified 15 virally encoded pre-miRNAs in both tumors, including all previously reported RRV pre-miRNAs. Although all 15 RRV pre-miRNAs, like all 12 KSHV pre-miRNAs, are located 3' to the conserved viral ORF71 gene and in the same transcriptional orientation, only one RRV miRNA is homologous to a KSHV miRNA. One previously identified RRV miRNA, miR-rR1-3, is actually a miRNA offset RNA (moRNA) derived from sequences located adjacent to pre-miR-rR1-3. Several other RRV-derived moRNAs were obtained, including one recovered > 600 times. Together, this research provides a comprehensive list of the miRNAs and moRNAs encoded by RRV.

Original languageEnglish (US)
Pages (from-to)592-599
Number of pages8
Issue number2
StatePublished - Sep 30 2010



  • Herpesviruses
  • Kaposi's sarcoma-associated herpesvirus
  • MicroRNAs
  • Post-transcriptional regulation
  • Rhesus rhadinovirus
  • Tumor virus

ASJC Scopus subject areas

  • Virology

Cite this