Analysis of naturally processed peptides eluted from HLA DRB1*0402 and *0404

James Hayden, A. L. McCormack, J. R. Yates, Michael Davey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Understanding the structural features of naturally processed peptides found within the major histocompatibility complex (MHC) class II peptide binding groove from disease-associated MHC molecules may provide insights into the nature of potential disease-related antigens. Class II MHC/peptide complexes were purified by immunoaffinity from transformed B cell lines homozygous for DRB1*0404 (an allele associated with rheumatoid arthritis) and *0402 (a closely related allele not associated with this disease). Peptides were eluted at acidic pH, fractionated by reversed phase HPLC, and analyzed by capillary electrophoresis. Those fractions containing a single dominant peptide were sequenced by automated Edman degradation and tandem mass spectrometry. The predominant peptide species identified came from non- polymorphic regions of the HLA class I molecules expressed by each cell line. Peptides from DRB1*0404 were found to be nested clusters derived from positions 26-43 of the HLA-B and -C α-chain. DRB1*0402 contained as the predominant peptide species a nested cluster from positions 129-145 of the HLA-B α-chain. The primary structure of the class I derived peptides was consistent with that seen by peptides exhibiting promiscuous DR binding behavior. Processing of MHC-derived peptides by MHC class II molecules is a common occurrence in the transformed B cell lines analyzed.

Original languageEnglish (US)
Pages (from-to)795-802
Number of pages8
JournalJournal of Neuroscience Research
Volume45
Issue number6
DOIs
StatePublished - Sep 15 1996

Fingerprint

Peptides
Major Histocompatibility Complex
Transformed Cell Line
HLA-B Antigens
HLA-DRB1*04:02 antigen
B-Lymphocytes
Alleles
HLA-C Antigens
Capillary Electrophoresis
Tandem Mass Spectrometry
Rheumatoid Arthritis
High Pressure Liquid Chromatography
Antigens
Cell Line

Keywords

  • indirect MHC presentation
  • MHC molecules
  • self-peptides

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Analysis of naturally processed peptides eluted from HLA DRB1*0402 and *0404. / Hayden, James; McCormack, A. L.; Yates, J. R.; Davey, Michael.

In: Journal of Neuroscience Research, Vol. 45, No. 6, 15.09.1996, p. 795-802.

Research output: Contribution to journalArticle

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