Analysis of molecular inversion probe performance for allele copy number determination

Yuker Wang; Martin Moorhead; George Karlin-Neumann; Nicholas J. Wang; James Ireland; Steven Lin; Chunnuan Chen; Laura M. Heiser; Koei Chin; Laura Esserman; Joe W. Gray; Paul T. Spellman; Malek Faham

doi:10.1186/gb-2007-8-11-r246

Analysis of molecular inversion probe performance for allele copy number determination

Yuker Wang, Martin Moorhead, George Karlin-Neumann, Nicholas J. Wang, James Ireland, Steven Lin, Chunnuan Chen, Laura M. Heiser, Koei Chin, Laura Esserman, Joe W. Gray, Paul T. Spellman, Malek Faham

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.

Original language	English (US)
Article number	R246
Journal	Genome biology
Volume	8
Issue number	11
DOIs	https://doi.org/10.1186/gb-2007-8-11-r246
State	Published - Nov 20 2007
Externally published	Yes

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Genetics
Cell Biology

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10.1186/gb-2007-8-11-r246

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author = "Yuker Wang and Martin Moorhead and George Karlin-Neumann and Wang, {Nicholas J.} and James Ireland and Steven Lin and Chunnuan Chen and Heiser, {Laura M.} and Koei Chin and Laura Esserman and Gray, {Joe W.} and Spellman, {Paul T.} and Malek Faham",

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AU - Wang, Yuker

AU - Moorhead, Martin

AU - Karlin-Neumann, George

AU - Wang, Nicholas J.

AU - Ireland, James

AU - Lin, Steven

AU - Chen, Chunnuan

AU - Heiser, Laura M.

AU - Chin, Koei

AU - Esserman, Laura

AU - Gray, Joe W.

AU - Spellman, Paul T.

AU - Faham, Malek

PY - 2007/11/20

Y1 - 2007/11/20

N2 - We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.

AB - We have developed a new protocol for using molecular inversion probes to accurately and specifically measure allele copy number. The new protocol provides for significant improvements, including the reduction of input DNA (from 2 μg) by more than 25-fold (to 75 ng total genomic DNA), higher overall precision resulting in one order of magnitude lower false positive rate, and greater dynamic range with accurate absolute copy number up to 60 copies.

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Analysis of molecular inversion probe performance for allele copy number determination

Abstract

ASJC Scopus subject areas

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