Analysis of mixed leucocyte culture (MLC) reactive cells after in vitro priming. Changes in avidity of T cell receptors

Reginald M. Gorczynski; Marvin B. Rittenberg

doi:10.1016/0008-8749(75)90196-3

Analysis of mixed leucocyte culture (MLC) reactive cells after in vitro priming. Changes in avidity of T cell receptors

Reginald M. Gorczynski, Marvin B. Rittenberg

Molecular Microbiology and Immunology

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6 Scopus citations

Abstract

Mixed leucocyte cultures were examined for populations of T cells responding to secondary stimulation with the priming antigen. Two such populations are described, one of which is stimulated optimally by low, the other by high doses of antigen. Both cell populations are sensitive to anti brain θ serum and complement, but are physically separable by size and by adherence on macrophage monolayers. The anti-brain θ-sensitive population stimulated by low antigen doses consists of larger cells with immunoglobulin-moieties on their surfaces.

Original language	English (US)
Pages (from-to)	171-181
Number of pages	11
Journal	Cellular Immunology
Volume	16
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(75)90196-3
State	Published - Mar 1975

ASJC Scopus subject areas

Immunology

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title = "Analysis of mixed leucocyte culture (MLC) reactive cells after in vitro priming. Changes in avidity of T cell receptors",

abstract = "Mixed leucocyte cultures were examined for populations of T cells responding to secondary stimulation with the priming antigen. Two such populations are described, one of which is stimulated optimally by low, the other by high doses of antigen. Both cell populations are sensitive to anti brain θ serum and complement, but are physically separable by size and by adherence on macrophage monolayers. The anti-brain θ-sensitive population stimulated by low antigen doses consists of larger cells with immunoglobulin-moieties on their surfaces.",

author = "Gorczynski, {Reginald M.} and Rittenberg, {Marvin B.}",

note = "Funding Information: 1 Supported by an Imperial Cancer Research Fund Grant (to N. A. M.) and by LrSPHS Grants (to M.B.R.) AM 13173 and C-4 12355 from the Sational Cancer Institute. 2 Author to Lvhom correspondence should be directed. Present address : Ontario (.{\textquoteleft}~nccr Institute, Toronto 5, Ontario, Canada. 3 Present address : Department of Microbiology, University 3181 SW. Sam Jackson Park Road, Portland, OR 97201.",

year = "1975",

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doi = "10.1016/0008-8749(75)90196-3",

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AU - Gorczynski, Reginald M.

AU - Rittenberg, Marvin B.

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N2 - Mixed leucocyte cultures were examined for populations of T cells responding to secondary stimulation with the priming antigen. Two such populations are described, one of which is stimulated optimally by low, the other by high doses of antigen. Both cell populations are sensitive to anti brain θ serum and complement, but are physically separable by size and by adherence on macrophage monolayers. The anti-brain θ-sensitive population stimulated by low antigen doses consists of larger cells with immunoglobulin-moieties on their surfaces.

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Analysis of mixed leucocyte culture (MLC) reactive cells after in vitro priming. Changes in avidity of T cell receptors

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