Analysis of immune cell infiltrates during squamous carcinoma development

Simon R. Junankar, Alexandra Eichten, Annegret Kramer, Karin E. De Visser, Lisa M. Coussens

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Infiltration of leukocytes into tissue is a common feature of many physiological and pathological conditions. Histopathologically, the diversity of leukocytes that infiltrate a tissue associated with a pathophysiologic response cannot be appreciated and/or examined unless highly selective immunologic detection methods are utilized. Specific populations of infiltrating leukocytes into squamous tissues harboring pre-malignant and/or malignant keratinocytes have recently been demonstrated to play a functionally significant role in the pathogenesis of squamous carcinomas. To evaluate immune cell types and quantify changes in their relative presence and localization during multi-stage neoplastic progression, we performed flow cytometry and histochemical detection using lineage-selective markers. Herein, we provide detailed methodology facilitating these analyses.

Original languageEnglish (US)
Pages (from-to)36-43
Number of pages8
JournalJournal of Investigative Dermatology Symposium Proceedings
Volume11
Issue number1
DOIs
StatePublished - Sep 1 2006

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Dermatology
  • Cell Biology

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