Analysis of IL-22 and Th22 cells by flow cytometry in systemic lupus erythematosus

Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, Cong Qiu Chu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Interleukin (IL)-22 belongs to the IL-10 family of cytokines. IL-22 exerts its biological effects via members of the cytokine receptor family class 2. CD4+ T helper (Th) cells predominantly producing IL-22 have been designated as Th22 cells. IL-22/Th22 cells are functionally related to IL-17/Th17 cells, but are distinctly different. Both IL-22 and IL-17 are cytokines recruiting neutrophils in response to microbe invasion. In chronic inflammation, IL-22 mediates protective and regenerative processes, whereas IL-17 cytokines tend to induce inflammation. Studies found that increased IL-22 levels and Th22 cells in peripheral blood were associated with disease activity in patients with systemic lupus erythematosus (SLE), but decreased IL-22 and Th22 cells were also reported. Here we describe analysis of IL-22 and Th22 cells in peripheral blood quantified by flow cytometry, and correlate our findings with SLE disease activity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages29-42
Number of pages14
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2108
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • ELISA
  • Flow cytometry
  • Interleukin-22
  • Systemic lupus erythematosus
  • Th22 cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Ye, Z., Zhao, L., Gao, Q., Jiang, Y., Jiang, Z., & Chu, C. Q. (2020). Analysis of IL-22 and Th22 cells by flow cytometry in systemic lupus erythematosus. In Methods in Molecular Biology (pp. 29-42). (Methods in Molecular Biology; Vol. 2108). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0247-8_3