Analysis of genetic relatedness in Gossypium species using RAPD

M. Gomes, G. Kulothungan, S. S. Mehetre, S. Eapen

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

RAPD markers were employed to assess genetic relatedness in seven Gossypium species, which included G. hirsutum CMS lines and cultivars. G. arboreum GMS lines, cultivars, and wild species, G. raimondii, G. bickii, G. thurberii, G. captis-viridis and G. anomalum, Out of 45 RAPD primers tested, 24 oligonucleotide primers yielded monomorphic amplified products or did not show any amplification product in some of the genotypes. The remaining 21 primers amplified a total of 168 fragments with an average of 9.8 fragments per primer. Out of the 11 genotypes studied, G. arboreum (G 27) produced the maximum number of DNA amplified fragments and G. raimondii produced the lowest number. On the basis of similarity coefficients cluster analysis was performed using UPGMA method. Cluster analysis resulted in 4 main cluster groups. Cluster one contained two subclusters IA and IB. Subcluster IA consisted of G. hirsutum lines, which represent AD genome. Subcluster IB contained G. arboreum genotypes with A genome. Cluster II consisted of G. raimodii, G. thurberii, which belong to the D genome. Cluster III consisted of only G. bickii representing the C genome and cluster 4 consisted of G. anomalum and G. captis-viridis representing the B genome. The clustering pattern obtained using RAPD analysis in the present study is in conformity with available information based on cytogenetic relationship.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalIndian Journal of Biotechnology
Volume3
Issue number1
StatePublished - Jan 2004
Externally publishedYes

Keywords

  • Genetic diversity
  • Gossypium species
  • RAPD markers

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Plant Science

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