Analysis of extracellular RNA in cerebrospinal fluid

Julie Saugstad, Theresa A. Lusardi, Kendall R. Van Keuren-Jensen, Jay I. Phillips, Babett Lind, Christina (Chris) Harrington, Trevor J. McFarland, Amanda L. Courtright, Rebecca A. Reiman, Ashish S. Yeri, M. Yashar S. Kalani, P. David Adelson, Jorge Arango, John P. Nolan, Erika Duggan, Karen Messer, Johnny C. Akers, Douglas R. Galasko, Joseph Quinn, Bob S. CarterFred H. Hochberg

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We examined the extracellular vesicle (EV) and RNA composition of pooled normal cerebrospinal fluid (CSF) samples and CSF from five major neurological disorders: Alzheimer’s disease (AD), Parkinson’s disease (PD), low-grade glioma (LGG), glioblastoma multiforme (GBM), and subarachnoid haemorrhage (SAH), representing neurodegenerative disease, cancer, and severe acute brain injury. We evaluated: (I) size and quantity of EVs by nanoparticle tracking analysis (NTA) and vesicle flow cytometry (VFC), (II) RNA yield and purity using four RNA isolation kits, (III) replication of RNA yields within and between laboratories, and (IV) composition of total and EV RNAs by reverse transcription–quantitative polymerase chain reaction (RT-qPCR) and RNA sequencing (RNASeq). The CSF contained ~106 EVs/μL by NTA and VFC. Brain tumour and SAH CSF contained more EVs and RNA relative to normal, AD, and PD. RT-qPCR and RNASeq identified disease-related populations of microRNAs and messenger RNAs (mRNAs) relative to normal CSF, in both total and EV fractions. This work presents relevant measures selected to inform the design of subsequent replicative CSF studies. The range of neurological diseases highlights variations in total and EV RNA content due to disease or collection site, revealing critical considerations guiding the selection of appropriate approaches and controls for CSF studies.

Original languageEnglish (US)
Article number1317577
JournalJournal of Extracellular Vesicles
Volume6
Issue number1
DOIs
StatePublished - Dec 1 2017

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Cerebrospinal Fluid
RNA
RNA Sequence Analysis
Subarachnoid Hemorrhage
Nanoparticles
Parkinson Disease
Flow Cytometry
Alzheimer Disease
Polymerase Chain Reaction
Intracranial Hemorrhages
Glioblastoma
Nervous System Diseases
MicroRNAs
Brain Neoplasms
Glioma
Neurodegenerative Diseases
Brain Injuries
Messenger RNA
Extracellular Vesicles
Population

Keywords

  • cerebrospinal fluid
  • extracellular RNA
  • Extracellular vesicles
  • neurological diseases

ASJC Scopus subject areas

  • Histology
  • Cell Biology

Cite this

Saugstad, J., Lusardi, T. A., Van Keuren-Jensen, K. R., Phillips, J. I., Lind, B., Harrington, C. C., ... Hochberg, F. H. (2017). Analysis of extracellular RNA in cerebrospinal fluid. Journal of Extracellular Vesicles, 6(1), [1317577]. https://doi.org/10.1080/20013078.2017.1317577

Analysis of extracellular RNA in cerebrospinal fluid. / Saugstad, Julie; Lusardi, Theresa A.; Van Keuren-Jensen, Kendall R.; Phillips, Jay I.; Lind, Babett; Harrington, Christina (Chris); McFarland, Trevor J.; Courtright, Amanda L.; Reiman, Rebecca A.; Yeri, Ashish S.; Kalani, M. Yashar S.; Adelson, P. David; Arango, Jorge; Nolan, John P.; Duggan, Erika; Messer, Karen; Akers, Johnny C.; Galasko, Douglas R.; Quinn, Joseph; Carter, Bob S.; Hochberg, Fred H.

In: Journal of Extracellular Vesicles, Vol. 6, No. 1, 1317577, 01.12.2017.

Research output: Contribution to journalArticle

Saugstad, J, Lusardi, TA, Van Keuren-Jensen, KR, Phillips, JI, Lind, B, Harrington, CC, McFarland, TJ, Courtright, AL, Reiman, RA, Yeri, AS, Kalani, MYS, Adelson, PD, Arango, J, Nolan, JP, Duggan, E, Messer, K, Akers, JC, Galasko, DR, Quinn, J, Carter, BS & Hochberg, FH 2017, 'Analysis of extracellular RNA in cerebrospinal fluid', Journal of Extracellular Vesicles, vol. 6, no. 1, 1317577. https://doi.org/10.1080/20013078.2017.1317577
Saugstad, Julie ; Lusardi, Theresa A. ; Van Keuren-Jensen, Kendall R. ; Phillips, Jay I. ; Lind, Babett ; Harrington, Christina (Chris) ; McFarland, Trevor J. ; Courtright, Amanda L. ; Reiman, Rebecca A. ; Yeri, Ashish S. ; Kalani, M. Yashar S. ; Adelson, P. David ; Arango, Jorge ; Nolan, John P. ; Duggan, Erika ; Messer, Karen ; Akers, Johnny C. ; Galasko, Douglas R. ; Quinn, Joseph ; Carter, Bob S. ; Hochberg, Fred H. / Analysis of extracellular RNA in cerebrospinal fluid. In: Journal of Extracellular Vesicles. 2017 ; Vol. 6, No. 1.
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