Analysis of DNA damage at the dinucleoside monophosphate level: Application to the formamido lesion

E. E. Budzinski, A. E. Maccubbin, M. S. Evans, Molly Kulesz-Martin, H. C. Box

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The dinucleoside monophosphates d(TpG), d(TpC), and d(TpT) were X- irradiated in oxygenated solution. In each case the modification of the dinucleoside in which the thymine base is degraded to a formamido remnant was observed as a principal product. The hydrolysis of the phosphoester bond of formamido-modified dinucleosides is much slower than that of the corresponding unmodified dinucleosides. This effect is also observable in the hydrolysis of irradiated DNA, where hydrolysis by nuclease P1 (plus acid phosphatase) generates the modified dinucleosides d(T(F)pN), T(F) being the modified thymidine. The total yield of the formamido lesion in all its forms, d(T(F)pN), exceeds the yield of any other base modification.

Original languageEnglish (US)
Pages (from-to)288-295
Number of pages8
JournalRadiation Research
Volume132
Issue number3
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Dinucleoside Phosphates
DNA damage
lesions
DNA Damage
hydrolysis
Hydrolysis
deoxyribonucleic acid
damage
nuclease
thymidine
thymine
phosphatases
Thymine
Deoxyribonucleases
nucleases
Acid Phosphatase
acid phosphatase
Thymidine
acids
DNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

Analysis of DNA damage at the dinucleoside monophosphate level : Application to the formamido lesion. / Budzinski, E. E.; Maccubbin, A. E.; Evans, M. S.; Kulesz-Martin, Molly; Box, H. C.

In: Radiation Research, Vol. 132, No. 3, 1992, p. 288-295.

Research output: Contribution to journalArticle

Budzinski, E. E. ; Maccubbin, A. E. ; Evans, M. S. ; Kulesz-Martin, Molly ; Box, H. C. / Analysis of DNA damage at the dinucleoside monophosphate level : Application to the formamido lesion. In: Radiation Research. 1992 ; Vol. 132, No. 3. pp. 288-295.
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