Analysis of connexin intracellular transport and assembly

Judy K. Vanslyke, Linda Musil

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Central to understanding the establishment and regulation of gap junction-mediated intercellular communication is a detailed knowledge of how these complex structures are assembled. In this article, we describe methods to modulate and/or monitor the transport of connexins from the endoplasmic reticulum to the Golgi complex and from the trans Golgi network to the cell surface. We also present techniques that we have developed to assay assembly of newly synthesized connexin monomers into connexons and gap junctional plaques. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)156-164
Number of pages9
JournalMethods
Volume20
Issue number2
DOIs
StatePublished - Feb 2000

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Connexins
trans-Golgi Network
Gap Junctions
Golgi Apparatus
Endoplasmic Reticulum
Assays
Monomers
Communication

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Analysis of connexin intracellular transport and assembly. / Vanslyke, Judy K.; Musil, Linda.

In: Methods, Vol. 20, No. 2, 02.2000, p. 156-164.

Research output: Contribution to journalArticle

Vanslyke, Judy K. ; Musil, Linda. / Analysis of connexin intracellular transport and assembly. In: Methods. 2000 ; Vol. 20, No. 2. pp. 156-164.
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