Anaemia stimulates aquaporin 1 expression in the fetal sheep heart

Sonnet Jonker, Lowell Davis, J. D W van der Bilt, B. Hadder, Alan (Roger) Hohimer, George Giraud, Kent Thornburg

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Interstitial fluid fluxes are much greater in the fetus than in the adult, and filtration rates are increased over control in most tissues of the anaemic fetus. Increased capillary filtration may lead to cardiac oedema which, in turn, severely impacts cardiac function. Mechanisms that underlie these differences in flux are incompletely understood. One possible mechanism is an increase in capillary water permeability. Therefore, the goal of our study was to determine the level of expression of the water channel aquaporin 1 (AQP1) during cardiac development and in the anaemic fetal sheep heart. Hearts from chronically instrumented anaemic sheep fetuses and hearts from normal early fetal, late fetal, neonatal and adult sheep were used for Northern and Western analyses and immunohistochemistry. We found that AQP1 mRNA levels were lower in the young fetal left ventricle than in the adult left ventricle (P <0.05). We also found that cardiac AQP1 expression was increased in anaemic fetuses compared to age-matched controls (P <0.05). Expression of AQP1 in all groups was greatest in the microvascular endothelium. These data suggest that AQP1 plays an important role in the physiological accommodation to fetal anaemia.

Original languageEnglish (US)
Pages (from-to)691-698
Number of pages8
JournalExperimental Physiology
Volume88
Issue number6
DOIs
StatePublished - Nov 2003

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Aquaporin 1
Fetal Heart
Anemia
Sheep
Fetus
Heart Ventricles
Cardiac Edema
Aquaporins
Extracellular Fluid
Capillary Permeability
Endothelium
Immunohistochemistry
Messenger RNA
Water

ASJC Scopus subject areas

  • Physiology

Cite this

Anaemia stimulates aquaporin 1 expression in the fetal sheep heart. / Jonker, Sonnet; Davis, Lowell; van der Bilt, J. D W; Hadder, B.; Hohimer, Alan (Roger); Giraud, George; Thornburg, Kent.

In: Experimental Physiology, Vol. 88, No. 6, 11.2003, p. 691-698.

Research output: Contribution to journalArticle

Jonker, Sonnet ; Davis, Lowell ; van der Bilt, J. D W ; Hadder, B. ; Hohimer, Alan (Roger) ; Giraud, George ; Thornburg, Kent. / Anaemia stimulates aquaporin 1 expression in the fetal sheep heart. In: Experimental Physiology. 2003 ; Vol. 88, No. 6. pp. 691-698.
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