An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST

Francesca Cargnin, Tamilla Nechiporuk, Karin Müllendorff, Deborah J. Stumpo, Perry J. Blackshear, Nurit Ballas, Gail Mandel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The RE1 Silencing Transcription Factor (REST) acts as a governor of the mature neuronal phenotype by repressing a large consortium of neuronal genes in non-neuronal cells. In the developing nervous system, REST is present in progenitors and downregulated at terminal differentiation to promote acquisition of mature neuronal phenotypes. Paradoxically, REST is still detected in some regions of the adult nervous system, but how REST levels are regulated, and whether REST can still repress neuronal genes, is not known. Here, we report that homeostatic levels of REST are maintained in mature peripheral neurons by a constitutive post-transcriptional mechanism. Specifically, using a three-hybrid genetic screen, we identify the RNA binding protein, ZFP36L2, associated previously only with female fertility and hematopoiesis, and show that it regulates REST mRNA stability. Dorsal root ganglia in Zfp36l2 knock-out mice, or wild-type ganglia expressing ZFP36L2 shRNA, show higher steady-state levels of Rest mRNA and protein, and extend thin and disintegrating axons. This phenotype is due, at least in part, to abnormally elevated REST levels in the ganglia because the axonal phenotype is attenuated by acute knockdown of REST in Zfp36l2 KO DRG explants. The higher REST levels result in lower levels of target genes, indicating that REST can still fine-tune gene expression through repression. Thus, REST levels are titrated in mature peripheral neurons, in part through a ZFP36L2-mediated post-transcriptional mechanism, with consequences for axonal integrity.

Original languageEnglish (US)
Pages (from-to)16650-16661
Number of pages12
JournalJournal of Neuroscience
Volume34
Issue number50
DOIs
StatePublished - Dec 10 2014

Fingerprint

RNA-Binding Proteins
Neurons
Phenotype
Ganglia
Nervous System
RE1-silencing transcription factor
Genes
Diagnosis-Related Groups
RNA Stability
Hematopoiesis
Spinal Ganglia
Knockout Mice
Small Interfering RNA
Fertility
Axons
Down-Regulation

Keywords

  • Axonal integrity
  • Peripheral nervous system
  • Post-transcriptional regulation
  • REST
  • RNA binding protein
  • ZFP36L2

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cargnin, F., Nechiporuk, T., Müllendorff, K., Stumpo, D. J., Blackshear, P. J., Ballas, N., & Mandel, G. (2014). An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST. Journal of Neuroscience, 34(50), 16650-16661. https://doi.org/10.1523/JNEUROSCI.1650-14.2014

An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST. / Cargnin, Francesca; Nechiporuk, Tamilla; Müllendorff, Karin; Stumpo, Deborah J.; Blackshear, Perry J.; Ballas, Nurit; Mandel, Gail.

In: Journal of Neuroscience, Vol. 34, No. 50, 10.12.2014, p. 16650-16661.

Research output: Contribution to journalArticle

Cargnin, F, Nechiporuk, T, Müllendorff, K, Stumpo, DJ, Blackshear, PJ, Ballas, N & Mandel, G 2014, 'An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST', Journal of Neuroscience, vol. 34, no. 50, pp. 16650-16661. https://doi.org/10.1523/JNEUROSCI.1650-14.2014
Cargnin F, Nechiporuk T, Müllendorff K, Stumpo DJ, Blackshear PJ, Ballas N et al. An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST. Journal of Neuroscience. 2014 Dec 10;34(50):16650-16661. https://doi.org/10.1523/JNEUROSCI.1650-14.2014
Cargnin, Francesca ; Nechiporuk, Tamilla ; Müllendorff, Karin ; Stumpo, Deborah J. ; Blackshear, Perry J. ; Ballas, Nurit ; Mandel, Gail. / An RNA binding protein promotes axonal integrity in peripheral neurons by destabilizing REST. In: Journal of Neuroscience. 2014 ; Vol. 34, No. 50. pp. 16650-16661.
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