An orthotopic murine model of human prostate cancer metastasis.

Janet Pavese, Irene M. Ogden, Raymond Bergan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Our laboratory has developed a novel orthotopic implantation model of human prostate cancer (PCa). As PCa death is not due to the primary tumor, but rather the formation of distinct metastasis, the ability to effectively model this progression pre-clinically is of high value. In this model, cells are directly implanted into the ventral lobe of the prostate in Balb/c athymic mice, and allowed to progress for 4-6 weeks. At experiment termination, several distinct endpoints can be measured, such as size and molecular characterization of the primary tumor, the presence and quantification of circulating tumor cells in the blood and bone marrow, and formation of metastasis to the lung. In addition to a variety of endpoints, this model provides a picture of a cells ability to invade and escape the primary organ, enter and survive in the circulatory system, and implant and grow in a secondary site. This model has been used effectively to measure metastatic response to both changes in protein expression as well as to response to small molecule therapeutics, in a short turnaround time.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number79
StatePublished - 2013
Externally publishedYes

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Prostatic Neoplasms
Neoplasm Metastasis
Circulating Neoplastic Cells
Tumors
Cardiovascular System
Osteogenesis
Nude Mice
Prostate
Neoplasms
Bone Marrow
Lung
Turnaround time
Proteins
Bone
Blood
Cells
Therapeutics
Molecules
Experiments

ASJC Scopus subject areas

  • Medicine(all)

Cite this

An orthotopic murine model of human prostate cancer metastasis. / Pavese, Janet; Ogden, Irene M.; Bergan, Raymond.

In: Journal of visualized experiments : JoVE, No. 79, 2013.

Research output: Contribution to journalArticle

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