An optimized method for cell-based phage display panning

Jennifer M. Watters, Pieter Telleman, Richard P. Junghans

    Research output: Contribution to journalArticle

    57 Scopus citations

    Abstract

    Background: Traditional methods of phage display panning, bind purified antigen to plates or other solid phases to which libraries are then applied, followed by vigorous washings in detergent-supplemented buffers to select for specific phage Fab. These methods are not directly applicable to antigens in their native environment on cell surfaces or in settings where the target antigen is unknown. Objectives: To develop a model antigen system employing whole cells rather than purified protein immobilized on a substrate; to optimize methods for phage display panning using a cell-based system. Results: Specificity of binding of phage Fab to antigen on cells was demonstrated by output titer and by flow cytometry. Output titers showed a plateau and binding advantage, after four washes, corresponding to removal of most non-specifically bound phage. Enrichment advantage was independent of input phage number. Longer incubation times, to cell tolerance, improved specific binding. Temperature had modest impact as a variable in the panning and washing. An increase in output titers paralleled enrichment for specific phage Fab. Conclusion: An optimized method applied to whole cells can productively enrich specific phage Fab in mixtures with large excesses of non-specific phage Fab over several rounds of panning.

    Original languageEnglish (US)
    Pages (from-to)21-29
    Number of pages9
    JournalImmunotechnology
    Volume3
    Issue number1
    DOIs
    StatePublished - Mar 1 1997

    Keywords

    • Autoantibody response
    • Cell surface antigen
    • Combinatorial library
    • Phage display

    ASJC Scopus subject areas

    • Immunology

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