An opsonic phagocytosis assay for plasmodium falciparum sporozoites

Ryan W.J. Steel, Brandon Wilder, Moriya Tsuji, Mary Jane L. Navarro, Will Betz, Matt E. Fishbaugher, Erika L. Flannery, Stefan H.I. Kappe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Plasmodium falciparum malaria remains the deadliest parasitic disease worldwide. Vaccines targeting the preerythrocytic sporozoite and liver stages have the potential to entirely prevent blood-stage infection and disease, as well as onward transmission. Sporozoite surface and secreted proteins are leading candidates for inclusion in a preerythrocytic stage-specific, antibody-based vaccine. Preclinical functional assays to identify humoral correlates of protection in vitro and to validate novel sporozoite protein targets for inclusion in multisubunit vaccines currently do not consider the interaction of sporozoite-targeting antibodies with other components of the immune system. Here, we describe the development of a simple flow cytometric assay to quantitatively assess the ability of antibodies directed against P. falciparum sporozoites to facilitate their phagocytosis. We demonstrate that this sporozoite opsonic phagocytosis assay (SOPA) is compatible with both monoclonal antibodies and human immune serum and can be performed using cryopreserved P. falciparum sporozoites. This simple, accessible assay will aid with the assessment of antibody responses to vaccination with Plasmodium antigens and their interaction with phagocytic cells of the immune system.

Original languageEnglish (US)
Article numbere00445
JournalClinical and Vaccine Immunology
Volume24
Issue number2
DOIs
StatePublished - Feb 1 2017
Externally publishedYes

Fingerprint

Sporozoites
Plasmodium falciparum
Phagocytosis
Assays
Vaccines
Antibodies
Immune system
Immune System
Liver
Immune Sera
Proteins
Blood
Monoclonal Antibodies
Parasitic Diseases
Plasmodium
Falciparum Malaria
Antigens
Phagocytes
Antibody Formation
Membrane Proteins

Keywords

  • Antibodies
  • Assay development
  • Malaria
  • Opsonization
  • Phagocytosis
  • Plasmodium falciparum
  • Sporozoite
  • Vaccines

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Steel, R. W. J., Wilder, B., Tsuji, M., Navarro, M. J. L., Betz, W., Fishbaugher, M. E., ... Kappe, S. H. I. (2017). An opsonic phagocytosis assay for plasmodium falciparum sporozoites. Clinical and Vaccine Immunology, 24(2), [e00445]. https://doi.org/10.1128/CVI.00445-16

An opsonic phagocytosis assay for plasmodium falciparum sporozoites. / Steel, Ryan W.J.; Wilder, Brandon; Tsuji, Moriya; Navarro, Mary Jane L.; Betz, Will; Fishbaugher, Matt E.; Flannery, Erika L.; Kappe, Stefan H.I.

In: Clinical and Vaccine Immunology, Vol. 24, No. 2, e00445, 01.02.2017.

Research output: Contribution to journalArticle

Steel, RWJ, Wilder, B, Tsuji, M, Navarro, MJL, Betz, W, Fishbaugher, ME, Flannery, EL & Kappe, SHI 2017, 'An opsonic phagocytosis assay for plasmodium falciparum sporozoites', Clinical and Vaccine Immunology, vol. 24, no. 2, e00445. https://doi.org/10.1128/CVI.00445-16
Steel, Ryan W.J. ; Wilder, Brandon ; Tsuji, Moriya ; Navarro, Mary Jane L. ; Betz, Will ; Fishbaugher, Matt E. ; Flannery, Erika L. ; Kappe, Stefan H.I. / An opsonic phagocytosis assay for plasmodium falciparum sporozoites. In: Clinical and Vaccine Immunology. 2017 ; Vol. 24, No. 2.
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