An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure

Ainhoa Plaza-Zabala, Xuan Li, Mike Milovanovic, Jessica A. Loweth, Rafael Maldonado, Fernando Berrendero, Marina Wolf

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hypocretin peptides are critical for the effects of cocaine on excitatory synaptic strength in the ventral tegmental area (VTA). However, little is known about their role in cocaine-induced synaptic plasticity in the nucleus accumbens (NAc). First, we tested whether hypocretin-1 by itself could acutely modulate glutamate receptor surface expression in the NAc, given that hypocretin-1 in the VTA reproduces cocaine's effects on glutamate transmission. We found no effect of hypocretin-1 infusion on AMPA or NMDA receptor surface expression in the NAc, measured by biotinylation, either 30. min or 3. h after the infusion. Second, we were interested in whether changes in hypocretin receptor-2 (Hcrtr-2) expression contribute to cocaine-induced plasticity in the NAc. As a first step towards addressing this question, Hcrtr-2 surface expression was compared in the NAc after withdrawal from extended-access self-administration of saline (control) versus cocaine. We found that surface Hcrtr-2 levels remain unchanged following 14, 25 or 48 days of withdrawal from cocaine, a time period in which high conductance GluA2-lacking AMPA receptors progressively emerge in the NAc. Overall, our results fail to support a role for hypocretins in acute modulation of glutamate receptor levels in the NAc or a role for altered Hcrtr-2 expression in withdrawal-dependent synaptic adaptations in the NAc following cocaine self-administration.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalNeuroscience Letters
Volume557
Issue numberPB
DOIs
StatePublished - Dec 17 2013
Externally publishedYes

Fingerprint

Orexin Receptors
Nucleus Accumbens
Glutamate Receptors
Cocaine
Ventral Tegmental Area
Self Administration
AMPA Receptors
Biotinylation
Neuronal Plasticity
Orexins
N-Methyl-D-Aspartate Receptors
Glutamic Acid
Peptides

Keywords

  • Cocaine
  • Glutamate receptor
  • Hypocretin
  • Nucleus accumbens
  • Orexin
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure. / Plaza-Zabala, Ainhoa; Li, Xuan; Milovanovic, Mike; Loweth, Jessica A.; Maldonado, Rafael; Berrendero, Fernando; Wolf, Marina.

In: Neuroscience Letters, Vol. 557, No. PB, 17.12.2013, p. 101-106.

Research output: Contribution to journalArticle

Plaza-Zabala, Ainhoa ; Li, Xuan ; Milovanovic, Mike ; Loweth, Jessica A. ; Maldonado, Rafael ; Berrendero, Fernando ; Wolf, Marina. / An investigation of interactions between hypocretin/orexin signaling and glutamate receptor surface expression in the rat nucleus accumbens under basal conditions and after cocaine exposure. In: Neuroscience Letters. 2013 ; Vol. 557, No. PB. pp. 101-106.
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