An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts

Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon Hennebold, Kyle E. Orwig, Amander T. Clark

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.

    Original languageEnglish (US)
    Pages (from-to)444-447
    Number of pages4
    JournalStem Cell Research
    Volume17
    Issue number2
    DOIs
    StatePublished - Sep 1 2016

    Fingerprint

    Virus Integration
    Induced Pluripotent Stem Cells
    Macaca mulatta
    Fibroblasts
    Cell Line
    Primates
    Embryonic Structures
    Teratoma
    Karyotype
    Transplantation
    Skin

    ASJC Scopus subject areas

    • Medicine(all)
    • Developmental Biology
    • Cell Biology

    Cite this

    An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. / Sosa, Enrique; Kim, Rachel; Rojas, Ernesto J.; Hosohama, Linzi; Hennebold, Jon; Orwig, Kyle E.; Clark, Amander T.

    In: Stem Cell Research, Vol. 17, No. 2, 01.09.2016, p. 444-447.

    Research output: Contribution to journalArticle

    Sosa, Enrique ; Kim, Rachel ; Rojas, Ernesto J. ; Hosohama, Linzi ; Hennebold, Jon ; Orwig, Kyle E. ; Clark, Amander T. / An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts. In: Stem Cell Research. 2016 ; Vol. 17, No. 2. pp. 444-447.
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    AU - Orwig, Kyle E.

    AU - Clark, Amander T.

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