An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts

Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon D. Hennebold, Kyle E. Orwig, Amander T. Clark

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.

Original languageEnglish (US)
Pages (from-to)444-447
Number of pages4
JournalStem Cell Research
Volume17
Issue number2
DOIs
StatePublished - Sep 2016

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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