An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Enrique Sosa; Rachel Kim; Ernesto J. Rojas; Linzi Hosohama; Jon D. Hennebold; Kyle E. Orwig; Amander T. Clark

doi:10.1016/j.scr.2017.03.011

An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon D. Hennebold, Kyle E. Orwig, Amander T. Clark

Oregon National Primate Research Center

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

Original language	English (US)
Pages (from-to)	5-8
Number of pages	4
Journal	Stem Cell Research
Volume	21
DOIs	https://doi.org/10.1016/j.scr.2017.03.011
State	Published - May 1 2017

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts",

abstract = "The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).",

author = "Enrique Sosa and Rachel Kim and Rojas, {Ernesto J.} and Linzi Hosohama and Hennebold, {Jon D.} and Orwig, {Kyle E.} and Clark, {Amander T.}",

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T1 - An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

AU - Sosa, Enrique

AU - Kim, Rachel

AU - Rojas, Ernesto J.

AU - Hosohama, Linzi

AU - Hennebold, Jon D.

AU - Orwig, Kyle E.

AU - Clark, Amander T.

PY - 2017/5/1

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AB - The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

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An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Abstract

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