An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Enrique Sosa, Rachel Kim, Ernesto J. Rojas, Linzi Hosohama, Jon Hennebold, Kyle E. Orwig, Amander T. Clark

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

    Original languageEnglish (US)
    Pages (from-to)5-8
    Number of pages4
    JournalStem Cell Research
    Volume21
    DOIs
    StatePublished - May 1 2017

    Fingerprint

    Virus Integration
    Induced Pluripotent Stem Cells
    Macaca mulatta
    Fibroblasts
    Cell Line
    Teratoma
    Karyotype
    Microsatellite Repeats
    Embryonic Structures
    Transplantation

    ASJC Scopus subject areas

    • Medicine(all)
    • Developmental Biology
    • Cell Biology

    Cite this

    An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts. / Sosa, Enrique; Kim, Rachel; Rojas, Ernesto J.; Hosohama, Linzi; Hennebold, Jon; Orwig, Kyle E.; Clark, Amander T.

    In: Stem Cell Research, Vol. 21, 01.05.2017, p. 5-8.

    Research output: Contribution to journalArticle

    Sosa, Enrique ; Kim, Rachel ; Rojas, Ernesto J. ; Hosohama, Linzi ; Hennebold, Jon ; Orwig, Kyle E. ; Clark, Amander T. / An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts. In: Stem Cell Research. 2017 ; Vol. 21. pp. 5-8.
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    AU - Hosohama, Linzi

    AU - Hennebold, Jon

    AU - Orwig, Kyle E.

    AU - Clark, Amander T.

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