An in vivo method to prepare normal Schwann cells free of axons and myelin

Peter Spencer, Harold J. Weinberg, Vivien Krygier-Brévart, Vivian Zabrenetzky

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

A viable population of undifferentiated Schwann cells may be prepared from chronically denervated peripheral nerves. Nerve transection stimulates a sequence of cellular events in distal stumps leading to removal of axons and myelin, and proliferation of Schwann cells. Sealing the ends of nerve stumps prevents reinnervation and leaves daughter Schwann cells residing in longitudinal columns. Schwann cells may be harvested from the endoneurial tissue of the nerve stumps 5-12 weeks after nerve transection. Unlike myelinating cells prepared from intact tissue, where function has been specified by associated axons, Schwann cells obtained from denervated stumps are functionally naive. Their usefulness in analyzing axonal regulation of myelinogenesis and mitosis is therefore suggested.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalBrain Research
Volume165
Issue number1
DOIs
StatePublished - Apr 6 1979
Externally publishedYes

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Schwann Cells
Myelin Sheath
Axons
Nerve Tissue
Peripheral Nerves
Mitosis
Population

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

An in vivo method to prepare normal Schwann cells free of axons and myelin. / Spencer, Peter; Weinberg, Harold J.; Krygier-Brévart, Vivien; Zabrenetzky, Vivian.

In: Brain Research, Vol. 165, No. 1, 06.04.1979, p. 119-126.

Research output: Contribution to journalArticle

Spencer, P, Weinberg, HJ, Krygier-Brévart, V & Zabrenetzky, V 1979, 'An in vivo method to prepare normal Schwann cells free of axons and myelin', Brain Research, vol. 165, no. 1, pp. 119-126. https://doi.org/10.1016/0006-8993(79)90049-0
Spencer, Peter ; Weinberg, Harold J. ; Krygier-Brévart, Vivien ; Zabrenetzky, Vivian. / An in vivo method to prepare normal Schwann cells free of axons and myelin. In: Brain Research. 1979 ; Vol. 165, No. 1. pp. 119-126.
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