@article{c0d73f0410454186b0be635bb214de6f,
title = "An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells",
abstract = "We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for in vivo cell transplantation studies. The protocol includes an enrichment step, making it useful for large-scale GMP manufacturing. RPE cells produced following this protocol are appropriate for cell replacement therapy for macular degeneration and disease modeling. For complete details on the use and execution of this protocol, please refer to Surendran et al. (2021).",
keywords = "Cell culture, Cell differentiation, Health sciences, Stem cells",
author = "Harshini Surendran and Lalitha Soundararajan and {Reddy K}, {Vijay Bhaskar} and Janavi Subramani and Jonathan Stoddard and Rene Reynaga and Wayne Tschetter and Ryals, {Renee C.} and Rajarshi Pal",
note = "Funding Information: Cellular and Molecular Platforms (C-CAMP), Bengaluru is acknowledged for their support in form of incubation and other core facilities. The authors acknowledge Swathi Narasimhaiah and Rashmi T. Prabhu for technical support and the Scientific Advisory Board for important suggestions during the course of optimizing and finalizing this protocol. H.S. and L.S. developed the RPE differentiation protocol and wrote the manuscript. H.S. L.S. V.B.R.K. and J.S. performed differentiation and characterization experiments. J.S. W.T. and R.R. performed animal experiments and did the analysis. R.R. and R.P. revised and proof-read the manuscript. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = dec,
day = "16",
doi = "10.1016/j.xpro.2022.101803",
language = "English (US)",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}