An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells

Harshini Surendran, Lalitha Soundararajan, Vijay Bhaskar Reddy K, Janavi Subramani, Jonathan Stoddard, Rene Reynaga, Wayne Tschetter, Renee C. Ryals, Rajarshi Pal

Research output: Contribution to journalArticlepeer-review

Abstract

We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for in vivo cell transplantation studies. The protocol includes an enrichment step, making it useful for large-scale GMP manufacturing. RPE cells produced following this protocol are appropriate for cell replacement therapy for macular degeneration and disease modeling. For complete details on the use and execution of this protocol, please refer to Surendran et al. (2021).

Original languageEnglish (US)
Article number101803
JournalSTAR Protocols
Volume3
Issue number4
DOIs
StatePublished - Dec 16 2022

Keywords

  • Cell culture
  • Cell differentiation
  • Health sciences
  • Stem cells

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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