An implantable tumor-window chamber model for the study of photodynamic therapy

S. J. Stern, S. Flock, S. Small, S. Thomsen, Steven Jacques

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In order to study both the anti-tumor effects and early vascular events in photodynamic therapy, a useful animal model has been developed. A window chamber is surgically placed on the dorsum of the Fischer-344 rat, and 500-μm fragments of the rat mammary adenocarcinoma 13672 are placed under direct vision into the subcutaneous tissue. Implantation of the chamber has been successfully completed in more than 50 rats. The operative procedure is straightforward and is accomplished in less than 1 hour. Using tumor fragments, tumor viability has been 60%. We have demonstrated obvious and reproducible neovascularization occurring as soon as 1 day after implantation. The application of this system to an experimental protocol comparing the photosensitizers dihematoporphyrin ether (DHE) and chloroaluminum sulfonated phthalocyanine (CASP) has yielded important information on early vascular events resulting from photodynamic therapy.

Original languageEnglish (US)
Pages (from-to)556-566
Number of pages11
JournalOtolaryngology - Head and Neck Surgery
Volume105
Issue number4
StatePublished - 1991
Externally publishedYes

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Photochemotherapy
Blood Vessels
Dihematoporphyrin Ether
Neoplasms
Photosensitizing Agents
Subcutaneous Tissue
Operative Surgical Procedures
Inbred F344 Rats
Adenocarcinoma
Breast
Animal Models

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Stern, S. J., Flock, S., Small, S., Thomsen, S., & Jacques, S. (1991). An implantable tumor-window chamber model for the study of photodynamic therapy. Otolaryngology - Head and Neck Surgery, 105(4), 556-566.

An implantable tumor-window chamber model for the study of photodynamic therapy. / Stern, S. J.; Flock, S.; Small, S.; Thomsen, S.; Jacques, Steven.

In: Otolaryngology - Head and Neck Surgery, Vol. 105, No. 4, 1991, p. 556-566.

Research output: Contribution to journalArticle

Stern, SJ, Flock, S, Small, S, Thomsen, S & Jacques, S 1991, 'An implantable tumor-window chamber model for the study of photodynamic therapy', Otolaryngology - Head and Neck Surgery, vol. 105, no. 4, pp. 556-566.
Stern, S. J. ; Flock, S. ; Small, S. ; Thomsen, S. ; Jacques, Steven. / An implantable tumor-window chamber model for the study of photodynamic therapy. In: Otolaryngology - Head and Neck Surgery. 1991 ; Vol. 105, No. 4. pp. 556-566.
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