An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase.

Ninian Blackburn, S. S. Hasnain, G. P. Diakun, P. F. Knowles, N. Binsted, C. D. Garner

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Copper and zinc K-edge-extended X-ray-absorption fine structures were measured for the metal sites of freeze-dried bovine superoxide dismutase and the model compounds tetrakis(imidazole)cupric nitrate and tetrakis(imidazole)zinc perchlorate. Detailed simulation of the spectra indicates that the copper site of the enzyme is best fit by co-ordination of four imidazole groups with Cu-N(alpha) distances of 0.198 nm (1.98 A). The zinc site is best fit by three imidazole groups at 0.201 nm (2.01 A) and an oxygen (from aspartate) at 0.203 nm (2.03 A).

Original languageEnglish (US)
Pages (from-to)765-768
Number of pages4
JournalBiochemical Journal
Volume213
Issue number3
StatePublished - Sep 1 1983
Externally publishedYes

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X ray absorption
Superoxide Dismutase
Zinc
Copper
X-Rays
Aspartic Acid
Metals
Oxygen
imidazole
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Blackburn, N., Hasnain, S. S., Diakun, G. P., Knowles, P. F., Binsted, N., & Garner, C. D. (1983). An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase. Biochemical Journal, 213(3), 765-768.

An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase. / Blackburn, Ninian; Hasnain, S. S.; Diakun, G. P.; Knowles, P. F.; Binsted, N.; Garner, C. D.

In: Biochemical Journal, Vol. 213, No. 3, 01.09.1983, p. 765-768.

Research output: Contribution to journalArticle

Blackburn, N, Hasnain, SS, Diakun, GP, Knowles, PF, Binsted, N & Garner, CD 1983, 'An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase.', Biochemical Journal, vol. 213, no. 3, pp. 765-768.
Blackburn, Ninian ; Hasnain, S. S. ; Diakun, G. P. ; Knowles, P. F. ; Binsted, N. ; Garner, C. D. / An extended X-ray-absorption-fine-structure study of the copper and zinc sites of freeze-dried bovine superoxide dismutase. In: Biochemical Journal. 1983 ; Vol. 213, No. 3. pp. 765-768.
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