An expression system to screen for inhibitors of parasite glucose transporters

Torben Feistel, Cheryl A. Hodson, David H. Peyton, Scott M. Landfear

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Chemotherapy of parasitic protists is limited by general toxicity, high expense and emergence of resistance to currently available drugs. Thus methods to identify new leads for further drug development are increasingly important. Previously, glucose transporters have been validated as new drug targets for protozoan parasites including Plasmodium falciparum, Leishmania mexicana and Trypanosoma brucei. A recently derived glucose transporter null mutant (Δlmgt) of L. mexicana was used to functionally express various heterologous glucose transporters including those from T. brucei THT1, P. falciparum PfHT and human GLUT1-resulting in recovery of growth of the Δlmgt null mutant in glucose replete medium. This heterologous expression system can be employed to screen for compounds that retard growth by inhibiting the expressed glucose transporter. The ability of this expression system to identify specific glucose transporter inhibitors was demonstrated using 3-O-undec-10-enyl-d-glucose, a previously described specific inhibitor of PfHT.

Original languageEnglish (US)
Pages (from-to)71-76
Number of pages6
JournalMolecular and Biochemical Parasitology
Volume162
Issue number1
DOIs
StatePublished - Nov 1 2008

Keywords

  • Glucose transporter
  • High-throughput screening
  • Leishmania mexicana
  • Null mutant
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

Fingerprint Dive into the research topics of 'An expression system to screen for inhibitors of parasite glucose transporters'. Together they form a unique fingerprint.

  • Cite this